Nodal regulates energy metabolism in glioma cells by inducing expression of hypoxia-inducible factor 1

Jing Huei Lai, Hsun Jin Jan, Li Wen Liu, Chin Cheng Lee, Shyang Guang Wang, Dueng Yuan Hueng, Yung Yen Cheng, Horng Mo Lee, Hsin I. Ma

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

BackgroundA shift in glucose metabolism from oxidative phosphorylation to anaerobic glycolysis is the biochemical hallmark of malignant cancer cells.MethodsIn the present study, we demonstrated that Nodal stimulated the expression of glycolytic enzymes and decreased reliance on mitochondrial oxidative phosphorylation in human glioma cancer cells. The shift in glucose metabolism was mediated by induction of the hypoxia-inducible factor (HIF).ResultsNodal protein expression was shown to be correlated with expression levels of glucose transporter (Glut)-1, hexokinase (HK)-II, pyruvate dehydrogenase kinase (PDK)-1, the phosphorylation level of pyruvate dehydrogenase (PDH), glucose uptake, and lactate accumulation in human glioma cells. These effects were inversely correlated with mitochondrial oxygen consumption and ATP production. Knockdown of Nodal expression with specific small hairpin RNA reduced Glut-1, HK-II, and PDK-1 expressions and PDH phosphorylation. Nodal knockdown also reduced glucose uptake and lactate generation, which in turn increased mitochondrial membrane potential (Ψ), O2 utilization, and ATP synthesis. The ectopic expression of Nodal in low-expressing Nodal glioma cells resulted in the opposite results compared with those of Nodal knockdown glioma cells. Treatment of cells with recombinant Nodal increased HIF-1 expression, and this effect was regulated at the transcriptional level. Blockage of the Nodal receptor by a pharmacological inhibitor or Nodal knockdown in U87MG cells decreased HIF-1 expression. Furthermore, HIF-1 knockdown in U87MG cells decreased Glut-1, HK-II, and PDK-1 expressions and PDH phosphorylation, which were similar to results in Nodal knockdown cells.ConclusionTaken together, these results suggest that Nodal affects energy metabolism through HIF-1.

Original languageEnglish
Pages (from-to)1330-1341
Number of pages12
JournalNeuro-Oncology
Volume15
Issue number10
DOIs
Publication statusPublished - Oct 2013

Fingerprint

Hypoxia-Inducible Factor 1
Glioma
Energy Metabolism
Hexokinase
Facilitative Glucose Transport Proteins
Pyruvic Acid
Oxidative Phosphorylation
Phosphorylation
Glucose
Oxidoreductases
Adenosine Triphosphate
Glucose 1-Dehydrogenase
Cell Hypoxia
Mitochondrial Membrane Potential
Glycolysis
L-Lactate Dehydrogenase
Oxygen Consumption
Small Interfering RNA
Lactic Acid
Neoplasms

Keywords

  • energy metabolism
  • gliomas
  • Glut-1
  • HIF-1
  • Nodal

ASJC Scopus subject areas

  • Cancer Research
  • Oncology
  • Clinical Neurology

Cite this

Lai, J. H., Jan, H. J., Liu, L. W., Lee, C. C., Wang, S. G., Hueng, D. Y., ... Ma, H. I. (2013). Nodal regulates energy metabolism in glioma cells by inducing expression of hypoxia-inducible factor 1. Neuro-Oncology, 15(10), 1330-1341. https://doi.org/10.1093/neuonc/not086

Nodal regulates energy metabolism in glioma cells by inducing expression of hypoxia-inducible factor 1. / Lai, Jing Huei; Jan, Hsun Jin; Liu, Li Wen; Lee, Chin Cheng; Wang, Shyang Guang; Hueng, Dueng Yuan; Cheng, Yung Yen; Lee, Horng Mo; Ma, Hsin I.

In: Neuro-Oncology, Vol. 15, No. 10, 10.2013, p. 1330-1341.

Research output: Contribution to journalArticle

Lai, JH, Jan, HJ, Liu, LW, Lee, CC, Wang, SG, Hueng, DY, Cheng, YY, Lee, HM & Ma, HI 2013, 'Nodal regulates energy metabolism in glioma cells by inducing expression of hypoxia-inducible factor 1', Neuro-Oncology, vol. 15, no. 10, pp. 1330-1341. https://doi.org/10.1093/neuonc/not086
Lai, Jing Huei ; Jan, Hsun Jin ; Liu, Li Wen ; Lee, Chin Cheng ; Wang, Shyang Guang ; Hueng, Dueng Yuan ; Cheng, Yung Yen ; Lee, Horng Mo ; Ma, Hsin I. / Nodal regulates energy metabolism in glioma cells by inducing expression of hypoxia-inducible factor 1. In: Neuro-Oncology. 2013 ; Vol. 15, No. 10. pp. 1330-1341.
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AU - Liu, Li Wen

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AU - Wang, Shyang Guang

AU - Hueng, Dueng Yuan

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AU - Lee, Horng Mo

AU - Ma, Hsin I.

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N2 - BackgroundA shift in glucose metabolism from oxidative phosphorylation to anaerobic glycolysis is the biochemical hallmark of malignant cancer cells.MethodsIn the present study, we demonstrated that Nodal stimulated the expression of glycolytic enzymes and decreased reliance on mitochondrial oxidative phosphorylation in human glioma cancer cells. The shift in glucose metabolism was mediated by induction of the hypoxia-inducible factor (HIF).ResultsNodal protein expression was shown to be correlated with expression levels of glucose transporter (Glut)-1, hexokinase (HK)-II, pyruvate dehydrogenase kinase (PDK)-1, the phosphorylation level of pyruvate dehydrogenase (PDH), glucose uptake, and lactate accumulation in human glioma cells. These effects were inversely correlated with mitochondrial oxygen consumption and ATP production. Knockdown of Nodal expression with specific small hairpin RNA reduced Glut-1, HK-II, and PDK-1 expressions and PDH phosphorylation. Nodal knockdown also reduced glucose uptake and lactate generation, which in turn increased mitochondrial membrane potential (Ψ), O2 utilization, and ATP synthesis. The ectopic expression of Nodal in low-expressing Nodal glioma cells resulted in the opposite results compared with those of Nodal knockdown glioma cells. Treatment of cells with recombinant Nodal increased HIF-1 expression, and this effect was regulated at the transcriptional level. Blockage of the Nodal receptor by a pharmacological inhibitor or Nodal knockdown in U87MG cells decreased HIF-1 expression. Furthermore, HIF-1 knockdown in U87MG cells decreased Glut-1, HK-II, and PDK-1 expressions and PDH phosphorylation, which were similar to results in Nodal knockdown cells.ConclusionTaken together, these results suggest that Nodal affects energy metabolism through HIF-1.

AB - BackgroundA shift in glucose metabolism from oxidative phosphorylation to anaerobic glycolysis is the biochemical hallmark of malignant cancer cells.MethodsIn the present study, we demonstrated that Nodal stimulated the expression of glycolytic enzymes and decreased reliance on mitochondrial oxidative phosphorylation in human glioma cancer cells. The shift in glucose metabolism was mediated by induction of the hypoxia-inducible factor (HIF).ResultsNodal protein expression was shown to be correlated with expression levels of glucose transporter (Glut)-1, hexokinase (HK)-II, pyruvate dehydrogenase kinase (PDK)-1, the phosphorylation level of pyruvate dehydrogenase (PDH), glucose uptake, and lactate accumulation in human glioma cells. These effects were inversely correlated with mitochondrial oxygen consumption and ATP production. Knockdown of Nodal expression with specific small hairpin RNA reduced Glut-1, HK-II, and PDK-1 expressions and PDH phosphorylation. Nodal knockdown also reduced glucose uptake and lactate generation, which in turn increased mitochondrial membrane potential (Ψ), O2 utilization, and ATP synthesis. The ectopic expression of Nodal in low-expressing Nodal glioma cells resulted in the opposite results compared with those of Nodal knockdown glioma cells. Treatment of cells with recombinant Nodal increased HIF-1 expression, and this effect was regulated at the transcriptional level. Blockage of the Nodal receptor by a pharmacological inhibitor or Nodal knockdown in U87MG cells decreased HIF-1 expression. Furthermore, HIF-1 knockdown in U87MG cells decreased Glut-1, HK-II, and PDK-1 expressions and PDH phosphorylation, which were similar to results in Nodal knockdown cells.ConclusionTaken together, these results suggest that Nodal affects energy metabolism through HIF-1.

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