Nocistatin excites rostral agranular insular cortex-periaqueductal gray projection neurons by enhancing transient receptor potential cation conductance via Gαq/11-PLC-protein kinase C pathway

Y. L. Chen, Ai Hsien Li, T. H. Yeh, An Hsun Chou, Y. S. Weng, H. L. Wang

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Rostral agranular insular cortex (RAIC) projects to periaqueductal gray (PAG) and inhibits spinal nociceptive transmission by activating PAG-rostral ventromedial medulla (RVM) descending antinociceptive circuitry. Despite being generated from the same precursor prepronociceptin, nocistatin (NST) and nociceptin/orphanin FQ (N/OFQ) produce supraspinal analgesic and hyperalgesic effects, respectively. Prepronociceptin is highly expressed in the RAIC. In the present study, we hypothesized that NST and N/OFQ modulate spinal pain transmission by regulating the activity of RAIC neurons projecting to ventrolateral PAG (RAIC-PAG). This hypothesis was tested by investigating electrophysiological effects of N/OFQ and NST on RAIC-PAG projection neurons in brain slice. Retrogradely labeled RAIC-PAG projection neurons are layer V pyramidal cells and express mRNA of vesicular glutamate transporter subtype 1, a marker for glutamatergic neurons. N/OFQ hyperpolarized 25% of RAIC-PAG pyramidal neurons by enhancing inwardly rectifying potassium conductance via pertussis toxin-sensitive Gαi/o. In contrast, NST depolarized 33% of RAIC-PAG glutamatergic neurons by causing the opening of canonical transient receptor potential (TRPC) cation channels through Gαq/11-phospholipase C-protein kinase C pathway. There were two separate populations of RAIC-PAG pyramidal neurons, one responding to NST and the other one to N/OFQ. Our results suggest that Gαq/11-coupled NST receptor mediates NST excitation of RAIC-PAG glutamatergic neurons, which is expected to cause the supraspinal analgesia by enhancing the activity of RAIC-PAG-RVM antinociceptive pathway. Opposite effects of NST and N/OFQ on supraspinal pain regulation are likely to result from their opposing effects on RAIC-PAG pyramidal neurons.

Original languageEnglish
Pages (from-to)226-239
Number of pages14
JournalNeuroscience
Volume168
Issue number1
DOIs
Publication statusPublished - Jun 2010
Externally publishedYes

Fingerprint

Periaqueductal Gray
Cerebral Cortex
Protein Kinase C
Cations
Neurons
Pyramidal Cells
TRPC Cation Channels
nocistatin
Vesicular Glutamate Transport Protein 1
Transient Receptor Potential Channels
Pain
Pertussis Toxin
Type C Phospholipases
Analgesia
Analgesics
nociceptin
Potassium

Keywords

  • Nociceptin/orphanin FQ
  • Nocistatin
  • Periaqueductal gray
  • Pyramidal neurons
  • Rostral agranular insular cortex
  • TRPC channel

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Nocistatin excites rostral agranular insular cortex-periaqueductal gray projection neurons by enhancing transient receptor potential cation conductance via Gαq/11-PLC-protein kinase C pathway. / Chen, Y. L.; Li, Ai Hsien; Yeh, T. H.; Chou, An Hsun; Weng, Y. S.; Wang, H. L.

In: Neuroscience, Vol. 168, No. 1, 06.2010, p. 226-239.

Research output: Contribution to journalArticle

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abstract = "Rostral agranular insular cortex (RAIC) projects to periaqueductal gray (PAG) and inhibits spinal nociceptive transmission by activating PAG-rostral ventromedial medulla (RVM) descending antinociceptive circuitry. Despite being generated from the same precursor prepronociceptin, nocistatin (NST) and nociceptin/orphanin FQ (N/OFQ) produce supraspinal analgesic and hyperalgesic effects, respectively. Prepronociceptin is highly expressed in the RAIC. In the present study, we hypothesized that NST and N/OFQ modulate spinal pain transmission by regulating the activity of RAIC neurons projecting to ventrolateral PAG (RAIC-PAG). This hypothesis was tested by investigating electrophysiological effects of N/OFQ and NST on RAIC-PAG projection neurons in brain slice. Retrogradely labeled RAIC-PAG projection neurons are layer V pyramidal cells and express mRNA of vesicular glutamate transporter subtype 1, a marker for glutamatergic neurons. N/OFQ hyperpolarized 25{\%} of RAIC-PAG pyramidal neurons by enhancing inwardly rectifying potassium conductance via pertussis toxin-sensitive Gαi/o. In contrast, NST depolarized 33{\%} of RAIC-PAG glutamatergic neurons by causing the opening of canonical transient receptor potential (TRPC) cation channels through Gαq/11-phospholipase C-protein kinase C pathway. There were two separate populations of RAIC-PAG pyramidal neurons, one responding to NST and the other one to N/OFQ. Our results suggest that Gαq/11-coupled NST receptor mediates NST excitation of RAIC-PAG glutamatergic neurons, which is expected to cause the supraspinal analgesia by enhancing the activity of RAIC-PAG-RVM antinociceptive pathway. Opposite effects of NST and N/OFQ on supraspinal pain regulation are likely to result from their opposing effects on RAIC-PAG pyramidal neurons.",
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AU - Li, Ai Hsien

AU - Yeh, T. H.

AU - Chou, An Hsun

AU - Weng, Y. S.

AU - Wang, H. L.

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