Nocistatin and nociceptin exert opposite effects on the excitability of central amygdala nucleus-periaqueductal gray projection neurons

Ying Ling Chen, Allen Hon Lun Li, Tu Hsueh Yeh, An Hsun Chou, Hung Li Wang

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Central amygdala nucleus (CeA)-periaqueductal gray (PAG) pathway is the component of descending antinociceptive circuitry. Nociceptin/orphanin FQ (N/OFQ) and nocistatin (NST) produce supraspinal pronociceptive and antinociceptive effects, respectively. We hypothesized that opposite effects of N/OFQ and NST on supraspinal pain modulation result from their opposing effects on the excitability of CeA-PAG projection neurons. This hypothesis was tested by investigating electrophysiological effects of N/OFQ and NST on medial CeA neurons that project to PAG (CeAM-PAG). N/OFQ hyperpolarized CeAM-PAG projection neurons by enhancing inwardly rectifying potassium conductance. In contrast, NST depolarized CeAM-PAG neurons by causing the opening of TRPC cation channels via Gαq/11-PLC-PKC pathway. CeAM-PAG neurons hyperpolarized by N/OFQ express CRF or neurotensin mRNA. NST-responsive CeAM-PAG neurons contain CRF or substance P mRNA. Our study provides the evidence that the molecular and cellular basis for opposite effects of N/OFQ and NST on supraspinal pain regulation is their opposing effects on the excitability of peptidergic CeAM-PAG neurons.

Original languageEnglish
Pages (from-to)76-88
Number of pages13
JournalMolecular and Cellular Neuroscience
Volume40
Issue number1
DOIs
Publication statusPublished - Jan 5 2009
Externally publishedYes

Fingerprint

Periaqueductal Gray
Neurons
TRPC Cation Channels
nocistatin
Central Amygdaloid Nucleus
nociceptin
Pain
Neurotensin
Messenger RNA
Substance P
Potassium

Keywords

  • CeA-PAG projection neurons
  • Central amygdala nucleus
  • Nociceptin/orphanin FQ
  • Nocistatin
  • Periaqueductal gray

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology
  • Cellular and Molecular Neuroscience

Cite this

Nocistatin and nociceptin exert opposite effects on the excitability of central amygdala nucleus-periaqueductal gray projection neurons. / Chen, Ying Ling; Li, Allen Hon Lun; Yeh, Tu Hsueh; Chou, An Hsun; Wang, Hung Li.

In: Molecular and Cellular Neuroscience, Vol. 40, No. 1, 05.01.2009, p. 76-88.

Research output: Contribution to journalArticle

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