NNOS-positive minor-branches of the dorsal penile nerves is associated with erectile function in the bilateral cavernous injury model of rats

Yen Lin Chen, Ting Ting Chao, Yi No Wu, Meng Chuan Chen, Ying Hung Lin, Chun Hou Liao, Chien Chih Wu, Kuo Chiang Chen, Shang Shing P. Chou, Han Sun Chiang

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

The changes in neuronal nitric oxide synthases (nNOS) in the dorsal penile nerves (DPNs) are consistent with cavernous nerve (CN) injury in rat models. However, the anatomical relationship and morphological changes between the minor branches of the DPNs and the CNs after injury have never been clearly explored. There were forty 12 week old male Sprague-Dawley rats receiving bilateral cavernous nerve injury (BCNI). Erectile function of intracavernous pressure and mean arterial pressure were measured. The histology and ultrastructure with H&E stain, Masson's trichrome stain and immunohistochemical stains were applied on the examination of CNs and DPNs. We demonstrated communicating nerve branches between the DPNs and the CNs in rats. The greatest damage and lowest erectile function were seen in the 14th day and partially recovered in the 28th day after BCNI. The nNOS positive DPN minor branches' number was significantly correlated with erectile function. The sub-analysis of the number of nNOS positive DPN minor branches also matched with the time course of the erectile function after BCNI. We suggest the regeneration of the DPNs minor branches would ameliorate the erectile function in BCNI rats.

Original languageEnglish
Article number18988
JournalScientific Reports
Volume8
Issue number1
DOIs
Publication statusPublished - Dec 1 2018

Fingerprint

Pudendal Nerve
Wounds and Injuries
Nitric Oxide Synthase Type I
Coloring Agents
Sprague Dawley Rats
Regeneration
Histology
Arterial Pressure
Pressure

ASJC Scopus subject areas

  • General

Cite this

NNOS-positive minor-branches of the dorsal penile nerves is associated with erectile function in the bilateral cavernous injury model of rats. / Chen, Yen Lin; Chao, Ting Ting; Wu, Yi No; Chen, Meng Chuan; Lin, Ying Hung; Liao, Chun Hou; Wu, Chien Chih; Chen, Kuo Chiang; Chou, Shang Shing P.; Chiang, Han Sun.

In: Scientific Reports, Vol. 8, No. 1, 18988, 01.12.2018.

Research output: Contribution to journalArticle

Chen, Yen Lin ; Chao, Ting Ting ; Wu, Yi No ; Chen, Meng Chuan ; Lin, Ying Hung ; Liao, Chun Hou ; Wu, Chien Chih ; Chen, Kuo Chiang ; Chou, Shang Shing P. ; Chiang, Han Sun. / NNOS-positive minor-branches of the dorsal penile nerves is associated with erectile function in the bilateral cavernous injury model of rats. In: Scientific Reports. 2018 ; Vol. 8, No. 1.
@article{2ec7c5673aa64359a5534930ba0868a1,
title = "NNOS-positive minor-branches of the dorsal penile nerves is associated with erectile function in the bilateral cavernous injury model of rats",
abstract = "The changes in neuronal nitric oxide synthases (nNOS) in the dorsal penile nerves (DPNs) are consistent with cavernous nerve (CN) injury in rat models. However, the anatomical relationship and morphological changes between the minor branches of the DPNs and the CNs after injury have never been clearly explored. There were forty 12 week old male Sprague-Dawley rats receiving bilateral cavernous nerve injury (BCNI). Erectile function of intracavernous pressure and mean arterial pressure were measured. The histology and ultrastructure with H&E stain, Masson's trichrome stain and immunohistochemical stains were applied on the examination of CNs and DPNs. We demonstrated communicating nerve branches between the DPNs and the CNs in rats. The greatest damage and lowest erectile function were seen in the 14th day and partially recovered in the 28th day after BCNI. The nNOS positive DPN minor branches' number was significantly correlated with erectile function. The sub-analysis of the number of nNOS positive DPN minor branches also matched with the time course of the erectile function after BCNI. We suggest the regeneration of the DPNs minor branches would ameliorate the erectile function in BCNI rats.",
author = "Chen, {Yen Lin} and Chao, {Ting Ting} and Wu, {Yi No} and Chen, {Meng Chuan} and Lin, {Ying Hung} and Liao, {Chun Hou} and Wu, {Chien Chih} and Chen, {Kuo Chiang} and Chou, {Shang Shing P.} and Chiang, {Han Sun}",
year = "2018",
month = "12",
day = "1",
doi = "10.1038/s41598-017-18988-2",
language = "English",
volume = "8",
journal = "Scientific Reports",
issn = "2045-2322",
publisher = "Nature Publishing Group",
number = "1",

}

TY - JOUR

T1 - NNOS-positive minor-branches of the dorsal penile nerves is associated with erectile function in the bilateral cavernous injury model of rats

AU - Chen, Yen Lin

AU - Chao, Ting Ting

AU - Wu, Yi No

AU - Chen, Meng Chuan

AU - Lin, Ying Hung

AU - Liao, Chun Hou

AU - Wu, Chien Chih

AU - Chen, Kuo Chiang

AU - Chou, Shang Shing P.

AU - Chiang, Han Sun

PY - 2018/12/1

Y1 - 2018/12/1

N2 - The changes in neuronal nitric oxide synthases (nNOS) in the dorsal penile nerves (DPNs) are consistent with cavernous nerve (CN) injury in rat models. However, the anatomical relationship and morphological changes between the minor branches of the DPNs and the CNs after injury have never been clearly explored. There were forty 12 week old male Sprague-Dawley rats receiving bilateral cavernous nerve injury (BCNI). Erectile function of intracavernous pressure and mean arterial pressure were measured. The histology and ultrastructure with H&E stain, Masson's trichrome stain and immunohistochemical stains were applied on the examination of CNs and DPNs. We demonstrated communicating nerve branches between the DPNs and the CNs in rats. The greatest damage and lowest erectile function were seen in the 14th day and partially recovered in the 28th day after BCNI. The nNOS positive DPN minor branches' number was significantly correlated with erectile function. The sub-analysis of the number of nNOS positive DPN minor branches also matched with the time course of the erectile function after BCNI. We suggest the regeneration of the DPNs minor branches would ameliorate the erectile function in BCNI rats.

AB - The changes in neuronal nitric oxide synthases (nNOS) in the dorsal penile nerves (DPNs) are consistent with cavernous nerve (CN) injury in rat models. However, the anatomical relationship and morphological changes between the minor branches of the DPNs and the CNs after injury have never been clearly explored. There were forty 12 week old male Sprague-Dawley rats receiving bilateral cavernous nerve injury (BCNI). Erectile function of intracavernous pressure and mean arterial pressure were measured. The histology and ultrastructure with H&E stain, Masson's trichrome stain and immunohistochemical stains were applied on the examination of CNs and DPNs. We demonstrated communicating nerve branches between the DPNs and the CNs in rats. The greatest damage and lowest erectile function were seen in the 14th day and partially recovered in the 28th day after BCNI. The nNOS positive DPN minor branches' number was significantly correlated with erectile function. The sub-analysis of the number of nNOS positive DPN minor branches also matched with the time course of the erectile function after BCNI. We suggest the regeneration of the DPNs minor branches would ameliorate the erectile function in BCNI rats.

UR - http://www.scopus.com/inward/record.url?scp=85041599954&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85041599954&partnerID=8YFLogxK

U2 - 10.1038/s41598-017-18988-2

DO - 10.1038/s41598-017-18988-2

M3 - Article

VL - 8

JO - Scientific Reports

JF - Scientific Reports

SN - 2045-2322

IS - 1

M1 - 18988

ER -