Nitric oxide inhibits androgen receptor-mediated collagen production in human gingival fibroblasts

S. J. Lin; H. K. Lu; H. W. Lee; Y. C. Chen; C. L. Li; Leng-Fang Wang

doi:10.1111/j.1600-0765.2012.01484.x

Nitric oxide inhibits androgen receptor-mediated collagen production in human gingival fibroblasts

S. J. Lin, H. K. Lu, H. W. Lee, Y. C. Chen, C. L. Li, Leng-Fang Wang

Research output: Contribution to journal › Article

9 Citations (Scopus)

Abstract

Background and Objective: In our previous study, we found that flutamide [an androgen receptor (AR) antagonist] inhibited the up-regulation of collagen induced by interleukin (IL)-1β and/or nifedipine in gingival fibroblasts. The present study attempted to verify the role of nitric oxide (NO) in the IL-1β/nifedipine-AR pathway in gingival overgrowth. Material and Methods: Confluent gingival fibroblasts derived from healthy individuals (n=4) and those with dihydropyridine-induced gingival overgrowth (DIGO) (n=6) were stimulated for 48h with IL-1β (10ng/mL), nifedipine (0.34μm) or IL-1β + nifedipine. Gene and protein expression were analyzed with real-time RT-PCR and western blot analyses, respectively. Meanwhile, Sircol dye-binding and the Griess reagent were, respectively, used to detect the concentrations of total soluble collagen and nitrite in the medium. Results: IL-1β and nifedipine simultaneously up-regulated the expression of the AR and type-I collagen α1 [Colα1(I)] genes and the total collagen concentration in DIGO cells (p

Original language	English
Pages (from-to)	701-710
Number of pages	10
Journal	Journal of Periodontal Research
Volume	47
Issue number	6
DOIs	https://doi.org/10.1111/j.1600-0765.2012.01484.x
Publication status	Published - Dec 2012

Keywords

Androgen receptor
Collagen
Interleukin-1β
Nifedipine
Nitric oxide

ASJC Scopus subject areas

Periodontics

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Lin, S. J., Lu, H. K., Lee, H. W., Chen, Y. C., Li, C. L., & Wang, L-F. (2012). Nitric oxide inhibits androgen receptor-mediated collagen production in human gingival fibroblasts. Journal of Periodontal Research, 47(6), 701-710. https://doi.org/10.1111/j.1600-0765.2012.01484.x

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abstract = "Background and Objective: In our previous study, we found that flutamide [an androgen receptor (AR) antagonist] inhibited the up-regulation of collagen induced by interleukin (IL)-1β and/or nifedipine in gingival fibroblasts. The present study attempted to verify the role of nitric oxide (NO) in the IL-1β/nifedipine-AR pathway in gingival overgrowth. Material and Methods: Confluent gingival fibroblasts derived from healthy individuals (n=4) and those with dihydropyridine-induced gingival overgrowth (DIGO) (n=6) were stimulated for 48h with IL-1β (10ng/mL), nifedipine (0.34μm) or IL-1β + nifedipine. Gene and protein expression were analyzed with real-time RT-PCR and western blot analyses, respectively. Meanwhile, Sircol dye-binding and the Griess reagent were, respectively, used to detect the concentrations of total soluble collagen and nitrite in the medium. Results: IL-1β and nifedipine simultaneously up-regulated the expression of the AR and type-I collagen α1 [Colα1(I)] genes and the total collagen concentration in DIGO cells (p",

keywords = "Androgen receptor, Collagen, Interleukin-1β, Nifedipine, Nitric oxide",

author = "Lin, {S. J.} and Lu, {H. K.} and Lee, {H. W.} and Chen, {Y. C.} and Li, {C. L.} and Leng-Fang Wang",

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AU - Lin, S. J.

AU - Lu, H. K.

AU - Lee, H. W.

AU - Chen, Y. C.

AU - Li, C. L.

AU - Wang, Leng-Fang

PY - 2012/12

Y1 - 2012/12

N2 - Background and Objective: In our previous study, we found that flutamide [an androgen receptor (AR) antagonist] inhibited the up-regulation of collagen induced by interleukin (IL)-1β and/or nifedipine in gingival fibroblasts. The present study attempted to verify the role of nitric oxide (NO) in the IL-1β/nifedipine-AR pathway in gingival overgrowth. Material and Methods: Confluent gingival fibroblasts derived from healthy individuals (n=4) and those with dihydropyridine-induced gingival overgrowth (DIGO) (n=6) were stimulated for 48h with IL-1β (10ng/mL), nifedipine (0.34μm) or IL-1β + nifedipine. Gene and protein expression were analyzed with real-time RT-PCR and western blot analyses, respectively. Meanwhile, Sircol dye-binding and the Griess reagent were, respectively, used to detect the concentrations of total soluble collagen and nitrite in the medium. Results: IL-1β and nifedipine simultaneously up-regulated the expression of the AR and type-I collagen α1 [Colα1(I)] genes and the total collagen concentration in DIGO cells (p

AB - Background and Objective: In our previous study, we found that flutamide [an androgen receptor (AR) antagonist] inhibited the up-regulation of collagen induced by interleukin (IL)-1β and/or nifedipine in gingival fibroblasts. The present study attempted to verify the role of nitric oxide (NO) in the IL-1β/nifedipine-AR pathway in gingival overgrowth. Material and Methods: Confluent gingival fibroblasts derived from healthy individuals (n=4) and those with dihydropyridine-induced gingival overgrowth (DIGO) (n=6) were stimulated for 48h with IL-1β (10ng/mL), nifedipine (0.34μm) or IL-1β + nifedipine. Gene and protein expression were analyzed with real-time RT-PCR and western blot analyses, respectively. Meanwhile, Sircol dye-binding and the Griess reagent were, respectively, used to detect the concentrations of total soluble collagen and nitrite in the medium. Results: IL-1β and nifedipine simultaneously up-regulated the expression of the AR and type-I collagen α1 [Colα1(I)] genes and the total collagen concentration in DIGO cells (p

KW - Androgen receptor

KW - Collagen

KW - Interleukin-1β

KW - Nifedipine

KW - Nitric oxide

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