Nitric oxide induces osteoblast apoptosis through the de novo synthesis of Bax protein

Ruei Ming Chen, Hwa Chang Liu, Yi Ling Lin, Wen Chyi Jean, Jui Shui Chen, Jyh Horng Wang

Research output: Contribution to journalArticle

42 Citations (Scopus)

Abstract

Nitric oxide (NO) plays a crucial role in the physiological and pathophysiological regulations of osteoblast functions. This study is designed to evaluate the toxic effects of NO released by sodium nitroprusside (SNP), an NO donor, on neonatal Wistar rat calvarial osteoblasts from the analyses of cell viability, alkaline phosphatase (ALP) activity, cell morphology, apoptotic cells, terminal deoxynucleotidyl transferase-mediated dUTP nick end-label (TUNEL) assay, DNA ladder, and immunocytochemistry and Western blot for proapoptotic Bax protein. SNP increased the levels of nitrite, an oxidative product of NO, in the culture medium of osteoblasts in concentration- and time-dependent manners, and altered cell morphologies to round and shrinkage shapes. Administration of osteoblasts with SNP resulted in concentration- and time-dependent decreases of cell viability and ALP activity. Analysis of apoptotic cells revealed that SNP increased the percentages of osteoblasts processing apoptosis. Analyses of TUNEL and DNA ladder showed that SNP caused DNA fragmentation. Pretreatment with cycloheximide, an inhibitor of protein synthesis, partially blocked SNP-induced osteoblast apoptosis. Imunocytochemical and immunoblotting analyses revealed that SNP increased Bax protein in osteoblasts. This study suggests that SNP could increase the levels of NO in osteoblasts, and cause osteoblast apoptosis possibly through the de novo synthesis of proapoptotic Bax protein.

Original languageEnglish
Pages (from-to)295-302
Number of pages8
JournalJournal of Orthopaedic Research
Volume20
Issue number2
DOIs
Publication statusPublished - 2002

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bcl-2-Associated X Protein
Osteoblasts
Nitroprusside
Nitric Oxide
Apoptosis
Alkaline Phosphatase
Cell Survival
Protein Synthesis Inhibitors
Nitric Oxide Donors
DNA Nucleotidylexotransferase
Poisons
DNA
DNA Fragmentation
Cycloheximide
Transferases
Nitrites
Immunoblotting
Culture Media
Wistar Rats
Western Blotting

ASJC Scopus subject areas

  • Orthopedics and Sports Medicine

Cite this

Nitric oxide induces osteoblast apoptosis through the de novo synthesis of Bax protein. / Chen, Ruei Ming; Liu, Hwa Chang; Lin, Yi Ling; Jean, Wen Chyi; Chen, Jui Shui; Wang, Jyh Horng.

In: Journal of Orthopaedic Research, Vol. 20, No. 2, 2002, p. 295-302.

Research output: Contribution to journalArticle

Chen, Ruei Ming ; Liu, Hwa Chang ; Lin, Yi Ling ; Jean, Wen Chyi ; Chen, Jui Shui ; Wang, Jyh Horng. / Nitric oxide induces osteoblast apoptosis through the de novo synthesis of Bax protein. In: Journal of Orthopaedic Research. 2002 ; Vol. 20, No. 2. pp. 295-302.
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