Nicotinamide abrogates acute lung injury caused by ischaemia/reperfusion

C. F. Su, D. D. Liu, S. J. Kao, H. I. Chen

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Poly (ADP-ribose) synthase or polymerase (PARS and PARP, respectively) is a cytotoxic enzyme which causes cellular damage. Nicotinamide, a compound of vitamin B complex, has been reported to exert an inhibitory effect on PARS or PARP. The present study tests the effects of nicotinamide on acute lung injury and associated alterations following ischaemia/reperfusion (I/R) of the isolated perfused rat's lung. I/R increased the lung weight (LW) to body weight ratio, LW gain, protein and dye tracer leakage, pulmonary arterial pressure and capillary permeability. The Insult also Increased nitrate/nitrite, methyl guanidine, tumour necrosis factor-α and interleukin-1β in lung perfusate, while it decreased adenosine triphosphate content with an increase in PARP activity in lung tissue. Most of the I/R-induced changes were abrogated by post-treatment (30 min after I/R) with nicotinamide (100 mg·kg-1 body weight). However, the increase in pulmonary arterial pressure was enhanced by nicotinamide post-treatment. Following I/R, the inducible nitric oxide synthase (INOS) mRNA expression was enhanced. Nicotinamide reduced the INOS expression. The results suggest that nicotinamide exerted a protective effect on the acute lung injury caused by ischaemia/reperfusion. The mechanisms may be mediated through the inhibition on the poly (adenosine diphosphate-ribose) polymerase activity, inducible nitric oxide synthase expression and the subsequent suppression of nitric oxide, free radicals and pro-inflammatory cytokines with restoration of adenosine triphosphate.

Original languageEnglish
Pages (from-to)199-204
Number of pages6
JournalEuropean Respiratory Journal
Volume30
Issue number2
DOIs
Publication statusPublished - Aug 2007

Keywords

  • Free radical
  • Inducible nitric oxide synthase
  • Nitric oxide
  • Poly (ADP-ribose) polymerase
  • Pro-inflammatory cytokines

ASJC Scopus subject areas

  • Pulmonary and Respiratory Medicine

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