New Hirsutinolide-Type Sesquiterpenoids from Vernonia cinerea Inhibit Nitric Oxide Production in LPS-Stimulated RAW264.7 Cells

Li Ming Yang Kuo, Pei Yi Tseng, Yu Chi Lin, Chia Ching Liaw, Li Jie Zhang, Keng Chang Tsai, Zhi Hu Lin, Hsiu O. Ho, Yao Haur Kuo

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Phytochemical investigation of ethanol extracts from two Taiwanese collections of Vernonia cinerea resulted in the isolation of eighteen hirsutinolide-type sesquiterpenoids, including seven new ones designated as vernolides E – K ( 1 – 7 ). All structures were determined by a combination of detailed spectroscopic analyses (NMR and MS) and comparison with reported data. In an in vitro anti-inflammatory assay, compounds 3, 7, 9, 11 , and 14 exhibited strong inhibitory activities toward NO production by LPS-induced RAW264.7 macrophages, with IC 50 values of 1.18, 0.85, 0.66, 0.71 and 0.45 µM, respectively, without affecting cellular viability at 40 µM. Preliminary structure-activity relationships indicate that the ester groups at C-8 and C-13 may enhance inhibition of NO production.

Original languageEnglish
JournalPlanta Medica
DOIs
Publication statusAccepted/In press - Jul 9 2018

Keywords

  • anti-inflammatory
  • Asteraceae
  • hirsutinolide
  • nitric oxide
  • RAW264.7 cells
  • vernolide
  • Vernonia cinerea

ASJC Scopus subject areas

  • Analytical Chemistry
  • Molecular Medicine
  • Pharmacology
  • Pharmaceutical Science
  • Drug Discovery
  • Complementary and alternative medicine
  • Organic Chemistry

Fingerprint Dive into the research topics of 'New Hirsutinolide-Type Sesquiterpenoids from Vernonia cinerea Inhibit Nitric Oxide Production in LPS-Stimulated RAW264.7 Cells'. Together they form a unique fingerprint.

  • Cite this

    Kuo, L. M. Y., Tseng, P. Y., Lin, Y. C., Liaw, C. C., Zhang, L. J., Tsai, K. C., Lin, Z. H., Ho, H. O., & Kuo, Y. H. (Accepted/In press). New Hirsutinolide-Type Sesquiterpenoids from Vernonia cinerea Inhibit Nitric Oxide Production in LPS-Stimulated RAW264.7 Cells. Planta Medica. https://doi.org/10.1055/a-0647-1901