New constituents with iNOS inhibitory activity from mycelium of Antrodia camphorata

Sien Sing Yang, Guei Jane Wang, Shiang Yi Wang, Yu Ying Lin, Yueh Hsiung Kuo, Zong-Huei Li

Research output: Contribution to journalArticle

40 Citations (Scopus)

Abstract

In continuing our investigation on the bioactive constituents ofmycelium of Antrodia camphorata, antroquinonol B (1), 4-acetyl-antroquinonol B (2), 2,3-(methylenedioxy)-6-methylbenzene-1,4-diol (3) and 2,4-dimethoxy-6- methylbenzene-1,3-diol (4) along with antrodin D (5) were isolated by the guidance of an inducible nitric oxide synthase (iNOS) inhibitory assay and identified on the basis of their spectroscopic analysis. The effect of these compounds on the inhibition of NO production in lipopolysaccharide (LPS)-activated murine macrophages was further evaluated. Compounds 4 and 5 significantly inhibited NO production without any cytotoxicity, the IC 50 values being 32.2 ± 0.1 and 26.3 ± 1.6 μ g/mL, respectively. Compounds 1 and 2 possessed greater effects on NO inhibition, with IC50 values of 16.2 ± 0.8 and 14.7 ± 2.8 μ g/mL, respectively, but displayed cytotoxicity at considerably higher concentrations. Compound 3 showed the lowest percent cell viability of 45.5 ± 1.8% as observed in treated cells at a concentration of 16.8 μ g/mL.

Original languageEnglish
Pages (from-to)512-516
Number of pages5
JournalPlanta Medica
Volume75
Issue number5
DOIs
Publication statusPublished - Apr 2009

Keywords

  • Antrodia camphorata
  • Antroquinonol B
  • iNOS
  • Mycelium
  • Polyporaceae

ASJC Scopus subject areas

  • Complementary and alternative medicine
  • Molecular Medicine
  • Organic Chemistry
  • Analytical Chemistry
  • Pharmaceutical Science
  • Pharmacology
  • Drug Discovery

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  • Cite this

    Yang, S. S., Wang, G. J., Wang, S. Y., Lin, Y. Y., Kuo, Y. H., & Li, Z-H. (2009). New constituents with iNOS inhibitory activity from mycelium of Antrodia camphorata. Planta Medica, 75(5), 512-516. https://doi.org/10.1055/s-0029-1185305