Neuroregenerative effects of BMP7 after stroke in rats

Jenny Chou, Brandon K. Harvey, Chen Fu Chang, Hui Shen, Marisela Morales, Yun Wang

Research output: Contribution to journalArticle

66 Citations (Scopus)

Abstract

Previous reports have indicated that the expression of bone morphogenetic protein-7 (BMP7) is enhanced after ischemic injury in brain. This upregulation may induce endogenous neurorepair in the ischemic brain. The purpose of this study was to examine neuroregenerative effects of BMP7 after ischemia-reperfusion injury. Adult Sprague-Dawley rats were anesthetized with chloral hydrate. Right middle cerebral artery (MCA) was transiently ligated with 10-O suture for 1 h. One day after MCA occlusion, vehicle or BMP7 was infused to the contralateral cerebral ventricle. To identify possible neurogenesis, bromodeoxyurindine (BrdU) was systemically injected on the fourth and fifth days after MCA occlusion. Animals treated with BMP7 showed a rapid correction of body asymmetry and neurological deficits, suggesting BMP7 facilitates recovery after stroke. Animals were sacrificed at 1 month after stroke and brains were analyzed using immunohistological techniques. BMP7 treatment enhanced immunoreactivity of BrdU in the subventricular zone, lesioned cortex, and corpus callosum. These BrdU-positive cells co-labeled with nestin and NeuN. Our behavioral and anatomical data suggest that BMP7 promotes neuroregeneration in stroke animals, possibly through the proliferation of new neuronal precursors after ischemia.

Original languageEnglish
Pages (from-to)21-29
Number of pages9
JournalJournal of the Neurological Sciences
Volume240
Issue number1-2
DOIs
Publication statusPublished - Jan 15 2006
Externally publishedYes

Keywords

  • Bone morphogenetic protein-7
  • Growth factors
  • Stroke

ASJC Scopus subject areas

  • Ageing
  • Clinical Neurology
  • Surgery
  • Developmental Neuroscience
  • Neurology
  • Neuroscience(all)

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  • Cite this

    Chou, J., Harvey, B. K., Chang, C. F., Shen, H., Morales, M., & Wang, Y. (2006). Neuroregenerative effects of BMP7 after stroke in rats. Journal of the Neurological Sciences, 240(1-2), 21-29. https://doi.org/10.1016/j.jns.2005.08.015