Neuroprotective effect of agmatine in rats with transient cerebral ischemia using MR imaging and histopathologic evaluation

Y. C. Huang, W. S. Tzeng, C. C. Wang, B. C. Cheng, Y. K. Chang, H. H. Chen, P. C. Lin, T. Y. Huang, T. J. Chuang, J. W. Lin, C. P. Chang

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Abstract

Purpose: This study aimed to further investigate the effects of agmatine on brain edema in the rats with middle cerebral artery occlusion (MCAO) injury using magnetic resonance imaging (MRI) monitoring and biochemical and histopathologic evaluation. Materials and methods: Following surgical induction of MCAO for 90. min, agmatine was injected 5. min after beginning of reperfusion and again once daily for the next 3 post-operative days. The events during ischemia and reperfusion were investigated by T2-weighted images (T2WI), serial diffusion-weighted images (DWI), calculated apparent diffusion coefficient (ADC) maps and contrast-enhanced T1-weighted images (CE-T1WI) during 3. h-72. h in a 1.5. T Siemens MAGNETON Avanto Scanner. Lesion volumes were analyzed in a blinded and randomized manner. Triphenyltetrazolium chloride (TTC), Nissl, and Evans Blue stainings were performed at the corresponding sections. Results: Increased lesion volumes derived from T2WI, DWI, ADC, CE-T1WI, and TTC all were noted at 3. h and peaked at 24. h-48. h after MCAO injury. TTC-derived infarct volumes were not significantly different from the T2WI, DWI-, and CE-T1WI-derived lesion volumes at the last imaging time (72. h) point except for significantly smaller ADC lesions in the MCAO model (P.

Original languageEnglish
Pages (from-to)1174-1181
Number of pages8
JournalMagnetic Resonance Imaging
Volume31
Issue number7
DOIs
Publication statusPublished - Sep 2013

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Keywords

  • Agmatine
  • Brain edema
  • Magnetic resonance image
  • Pharmacology
  • Stroke

ASJC Scopus subject areas

  • Biophysics
  • Radiology Nuclear Medicine and imaging
  • Biomedical Engineering

Cite this

Huang, Y. C., Tzeng, W. S., Wang, C. C., Cheng, B. C., Chang, Y. K., Chen, H. H., Lin, P. C., Huang, T. Y., Chuang, T. J., Lin, J. W., & Chang, C. P. (2013). Neuroprotective effect of agmatine in rats with transient cerebral ischemia using MR imaging and histopathologic evaluation. Magnetic Resonance Imaging, 31(7), 1174-1181. https://doi.org/10.1016/j.mri.2013.03.026