Neuroprotection and free radical scavenging effects of Osmanthus fragrans

Hsin Hsueh Lee, Chi Ting Lin, Ling Ling Yang

Research output: Contribution to journalArticle

49 Citations (Scopus)

Abstract

The ethanol extract of dried flowers Osmanthus fragrans (OFE) was assessed for free radical scavenging effects measured by the bleaching of the 1,1-diphenyl-2-picryl-hydrazyl (DPPH) radical, scavenging of the hydroxyl anion, investigation of the ferric reducing/antioxidant power (FRAP) and lipid-peroxidation inhibition in rat tissues. OFE contained a high amount of total flavonoid and polyphenol. OFE presented the effects in the metal reducing power, FRAP assay with IC50 values of 0.23 μg/ml, and 7.74 μg/ml, respectively. OFE presented similar activities toward the DPPH and hydroxyl anion scavenging ability with IC50 values of 10 μg/ml. OFE with IC50 values between 46 and 97 μg/ml inhibited lipid peroxidation initiated by ferrous chloride in rat brain, liver, heart and kidney mitochodrias. Moreover, the neuroprotective activity of OFE was investigated under different insults (glutamate, arachidonic acid, and 6-hydroxydopamine) in Wistar rat primary cortical neurons. OFE with EC50 values between 66 and 165 μg/ml attenuated the neurotoxicity on MTT and LDH assays. In addition, the AKT protein expression of excitotoxicity and oxidative stress was displayed by western blotting analysis. OFE could up-regulate the glutamate and 6-OHDA decreased AKT expression. This is the first demonstration of the neuroprotective, free radical scavenging and anti-oxidative effects of O. fragrans.

Original languageEnglish
Pages (from-to)819-827
Number of pages9
JournalJournal of Biomedical Science
Volume14
Issue number6
DOIs
Publication statusPublished - Nov 2007

Fingerprint

Scavenging
Inhibitory Concentration 50
Free Radicals
Oxidopamine
Rats
Hydroxyl Radical
Lipid Peroxidation
Anions
Glutamic Acid
Antioxidants
Assays
Polyphenols
Lipids
Flavonoids
Arachidonic Acid
Oxidative stress
Wistar Rats
Oxidative Stress
Ethanol
Up-Regulation

Keywords

  • Free radical scavenging
  • Neuroprotection
  • Osmanthus fragrans
  • Oxidative stress
  • Primary cortical neuron

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Neuroprotection and free radical scavenging effects of Osmanthus fragrans. / Lee, Hsin Hsueh; Lin, Chi Ting; Yang, Ling Ling.

In: Journal of Biomedical Science, Vol. 14, No. 6, 11.2007, p. 819-827.

Research output: Contribution to journalArticle

Lee, Hsin Hsueh ; Lin, Chi Ting ; Yang, Ling Ling. / Neuroprotection and free radical scavenging effects of Osmanthus fragrans. In: Journal of Biomedical Science. 2007 ; Vol. 14, No. 6. pp. 819-827.
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