Neurocytoprotective effects of the bioactive constituents of Pueraria thomsonii in 6-hydroxydopamine (6-OHDA)-treated nerve growth factor (NGF)-differentiated PC12 cells

Chien Min Lin, Rong Dih Lin, Shui Tein Chen, Yi Pei Lin, Wen Ta Chiu, Jia Wei Lin, Feng Lin Hsu, Mei Hsien Lee

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25 Citations (Scopus)

Abstract

Chronic neurodegenerative disorders are having an increasing impact on public health as human longevity increases. Parkinson's disease (PD) is a degenerative disorder of the central nervous system and is characterized by motor system disorders resulting in loss of dopamine-producing brain cells. Pueraria thomsonii Benth. (Fabaceae) is an herbal medicine that has traditionally been used as an antipyretic agent. In the present study, the active constituents, daidzein and genistein, were isolated from P. thomsonii. Both compounds exhibited neurocytoprotective effects against 6-hydroxydopamine (6-OHDA)-induced cytotoxicity in nerve growth factor (NGF)-differentiated PC12 cells. Neither daidzein nor genistein affected 6-OHDA-induced cellular reactive oxygen species (ROS) generation according to flow cytometric analysis. Rather, they inhibited caspase-8 and partially inhibited caspase-3 activation, providing a protective mechanism against 6-OHDA-induced cytotoxicity in NGF-differentiated PC12 cells. The present results imply that daidzein and genistein may be useful in the development of future strategies for the treatment of PD.

Original languageEnglish
Pages (from-to)2147-2156
Number of pages10
JournalPhytochemistry
Volume71
Issue number17-18
DOIs
Publication statusPublished - Dec 2010

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Keywords

  • 6-Hydroxydopamine
  • Daidzein
  • Fabaceae
  • Genistein
  • Neurocytoprotection
  • PC12 cells
  • Pueraria thomsonii Benth.

ASJC Scopus subject areas

  • Plant Science
  • Biochemistry
  • Molecular Biology
  • Horticulture

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