24 Citations (Scopus)

Abstract

Chronic neurodegenerative disorders are having an increasing impact on public health as human longevity increases. Parkinson's disease (PD) is a degenerative disorder of the central nervous system and is characterized by motor system disorders resulting in loss of dopamine-producing brain cells. Pueraria thomsonii Benth. (Fabaceae) is an herbal medicine that has traditionally been used as an antipyretic agent. In the present study, the active constituents, daidzein and genistein, were isolated from P. thomsonii. Both compounds exhibited neurocytoprotective effects against 6-hydroxydopamine (6-OHDA)-induced cytotoxicity in nerve growth factor (NGF)-differentiated PC12 cells. Neither daidzein nor genistein affected 6-OHDA-induced cellular reactive oxygen species (ROS) generation according to flow cytometric analysis. Rather, they inhibited caspase-8 and partially inhibited caspase-3 activation, providing a protective mechanism against 6-OHDA-induced cytotoxicity in NGF-differentiated PC12 cells. The present results imply that daidzein and genistein may be useful in the development of future strategies for the treatment of PD.

Original languageEnglish
Pages (from-to)2147-2156
Number of pages10
JournalPhytochemistry
Volume71
Issue number17-18
DOIs
Publication statusPublished - Dec 2010

Fingerprint

Pueraria montana var. thomsonii
Pueraria
nerve growth factor
daidzein
Oxidopamine
PC12 Cells
genistein
Nerve Growth Factor
Genistein
Parkinson disease
cytotoxicity
Cytotoxicity
antipyretics
Parkinson Disease
caspase-8
herbal medicines
neurodegenerative diseases
cells
caspase-3
dopamine

Keywords

  • 6-Hydroxydopamine
  • Daidzein
  • Fabaceae
  • Genistein
  • Neurocytoprotection
  • PC12 cells
  • Pueraria thomsonii Benth.

ASJC Scopus subject areas

  • Plant Science
  • Biochemistry
  • Molecular Biology
  • Horticulture

Cite this

Neurocytoprotective effects of the bioactive constituents of Pueraria thomsonii in 6-hydroxydopamine (6-OHDA)-treated nerve growth factor (NGF)-differentiated PC12 cells. / Lin, Chien Min; Lin, Rong Dih; Chen, Shui Tein; Lin, Yi Pei; Chiu, Wen Ta; Lin, Jia Wei; Hsu, Feng Lin; Lee, Mei Hsien.

In: Phytochemistry, Vol. 71, No. 17-18, 12.2010, p. 2147-2156.

Research output: Contribution to journalArticle

@article{614c2e983f6541aa9428a60074f33273,
title = "Neurocytoprotective effects of the bioactive constituents of Pueraria thomsonii in 6-hydroxydopamine (6-OHDA)-treated nerve growth factor (NGF)-differentiated PC12 cells",
abstract = "Chronic neurodegenerative disorders are having an increasing impact on public health as human longevity increases. Parkinson's disease (PD) is a degenerative disorder of the central nervous system and is characterized by motor system disorders resulting in loss of dopamine-producing brain cells. Pueraria thomsonii Benth. (Fabaceae) is an herbal medicine that has traditionally been used as an antipyretic agent. In the present study, the active constituents, daidzein and genistein, were isolated from P. thomsonii. Both compounds exhibited neurocytoprotective effects against 6-hydroxydopamine (6-OHDA)-induced cytotoxicity in nerve growth factor (NGF)-differentiated PC12 cells. Neither daidzein nor genistein affected 6-OHDA-induced cellular reactive oxygen species (ROS) generation according to flow cytometric analysis. Rather, they inhibited caspase-8 and partially inhibited caspase-3 activation, providing a protective mechanism against 6-OHDA-induced cytotoxicity in NGF-differentiated PC12 cells. The present results imply that daidzein and genistein may be useful in the development of future strategies for the treatment of PD.",
keywords = "6-Hydroxydopamine, Daidzein, Fabaceae, Genistein, Neurocytoprotection, PC12 cells, Pueraria thomsonii Benth.",
author = "Lin, {Chien Min} and Lin, {Rong Dih} and Chen, {Shui Tein} and Lin, {Yi Pei} and Chiu, {Wen Ta} and Lin, {Jia Wei} and Hsu, {Feng Lin} and Lee, {Mei Hsien}",
year = "2010",
month = "12",
doi = "10.1016/j.phytochem.2010.08.015",
language = "English",
volume = "71",
pages = "2147--2156",
journal = "Phytochemistry",
issn = "0031-9422",
publisher = "Elsevier Limited",
number = "17-18",

}

TY - JOUR

T1 - Neurocytoprotective effects of the bioactive constituents of Pueraria thomsonii in 6-hydroxydopamine (6-OHDA)-treated nerve growth factor (NGF)-differentiated PC12 cells

AU - Lin, Chien Min

AU - Lin, Rong Dih

AU - Chen, Shui Tein

AU - Lin, Yi Pei

AU - Chiu, Wen Ta

AU - Lin, Jia Wei

AU - Hsu, Feng Lin

AU - Lee, Mei Hsien

PY - 2010/12

Y1 - 2010/12

N2 - Chronic neurodegenerative disorders are having an increasing impact on public health as human longevity increases. Parkinson's disease (PD) is a degenerative disorder of the central nervous system and is characterized by motor system disorders resulting in loss of dopamine-producing brain cells. Pueraria thomsonii Benth. (Fabaceae) is an herbal medicine that has traditionally been used as an antipyretic agent. In the present study, the active constituents, daidzein and genistein, were isolated from P. thomsonii. Both compounds exhibited neurocytoprotective effects against 6-hydroxydopamine (6-OHDA)-induced cytotoxicity in nerve growth factor (NGF)-differentiated PC12 cells. Neither daidzein nor genistein affected 6-OHDA-induced cellular reactive oxygen species (ROS) generation according to flow cytometric analysis. Rather, they inhibited caspase-8 and partially inhibited caspase-3 activation, providing a protective mechanism against 6-OHDA-induced cytotoxicity in NGF-differentiated PC12 cells. The present results imply that daidzein and genistein may be useful in the development of future strategies for the treatment of PD.

AB - Chronic neurodegenerative disorders are having an increasing impact on public health as human longevity increases. Parkinson's disease (PD) is a degenerative disorder of the central nervous system and is characterized by motor system disorders resulting in loss of dopamine-producing brain cells. Pueraria thomsonii Benth. (Fabaceae) is an herbal medicine that has traditionally been used as an antipyretic agent. In the present study, the active constituents, daidzein and genistein, were isolated from P. thomsonii. Both compounds exhibited neurocytoprotective effects against 6-hydroxydopamine (6-OHDA)-induced cytotoxicity in nerve growth factor (NGF)-differentiated PC12 cells. Neither daidzein nor genistein affected 6-OHDA-induced cellular reactive oxygen species (ROS) generation according to flow cytometric analysis. Rather, they inhibited caspase-8 and partially inhibited caspase-3 activation, providing a protective mechanism against 6-OHDA-induced cytotoxicity in NGF-differentiated PC12 cells. The present results imply that daidzein and genistein may be useful in the development of future strategies for the treatment of PD.

KW - 6-Hydroxydopamine

KW - Daidzein

KW - Fabaceae

KW - Genistein

KW - Neurocytoprotection

KW - PC12 cells

KW - Pueraria thomsonii Benth.

UR - http://www.scopus.com/inward/record.url?scp=78249279052&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=78249279052&partnerID=8YFLogxK

U2 - 10.1016/j.phytochem.2010.08.015

DO - 10.1016/j.phytochem.2010.08.015

M3 - Article

C2 - 20832831

AN - SCOPUS:78249279052

VL - 71

SP - 2147

EP - 2156

JO - Phytochemistry

JF - Phytochemistry

SN - 0031-9422

IS - 17-18

ER -