Neuroactive steroids inhibit spinal reflex potentiation by selectively enhancing specific spinal GABAA receptor subtypes

Hsien Yu Peng, Gin Den Chen, Shin Da Lee, Cheng Yuan Lai, Chun Hsien Chiu, Chen Li Cheng, Yu Shuo Chang, Ming Chun Hsieh, Kwong Chung Tung, Tzer Bin Lin

Research output: Contribution to journalArticle

39 Citations (Scopus)

Abstract

Recently, we demonstrated a spinal GABAA receptor (GABAAR)-dependent inhibition on the induction of repetitive stimulation-induced spinal reflex potentiation. However, it remains unclear whether steroid hormones modulate such an inhibition. Here, we show that progesterone is capable of producing GABAARs-dependent inhibition of the induction of spinal reflex potentiation by actions through neurosteroid metabolites. Progesterone (5 mg/kg, twice daily for 4 days) up-regulates the expression of GABAAR α2, α3, α4 and δ subunits, and is associated with attenuated repetitive stimulation-induced spinal reflex activity in ovariectomized rats. These changes were blocked by finasteride (50 mg/kg, twice daily), an antagonist of neurosteroid synthesis from progesterone, but not by the progesterone receptor antagonist, RU486 (100 mg/kg, twice daily). The induction of spinal reflex potentiation was attenuated after a short (30 min) intrathecal treatment with the neurosteroids, allopregnanolone (ALLOP, 10 μM, 10 μL) and 3α,5α-tetrahydrodeoxycorticosterone (THDOC, 10 μM, 10 μL). Acute intrathecal administration of the GABAAR antagonist, bicuculline (10 μM, 10 μL) reversed the inhibition produced by progesterone, THDOC and allopregnanolone. These results imply that progesterone-mediated effects on GABAAR expression and neural inhibition are regulated by neurosteroids synthesis rather than progesterone receptor activation.

Original languageEnglish
Pages (from-to)12-20
Number of pages9
JournalPain
Volume143
Issue number1-2
DOIs
Publication statusPublished - May 2009
Externally publishedYes

Fingerprint

GABA-A Receptors
Progesterone
Reflex
Steroids
Neurotransmitter Agents
Pregnanolone
Progesterone Receptors
Neural Inhibition
Finasteride
GABA-A Receptor Antagonists
Bicuculline
Up-Regulation
Hormones
Inhibition (Psychology)
tetrahydrodeoxycorticosterone

Keywords

  • Estrus cycle
  • Hyperalgesia
  • Pain
  • Progesterone
  • Spinal cord

ASJC Scopus subject areas

  • Clinical Neurology
  • Anesthesiology and Pain Medicine
  • Neurology
  • Pharmacology

Cite this

Neuroactive steroids inhibit spinal reflex potentiation by selectively enhancing specific spinal GABAA receptor subtypes. / Peng, Hsien Yu; Chen, Gin Den; Lee, Shin Da; Lai, Cheng Yuan; Chiu, Chun Hsien; Cheng, Chen Li; Chang, Yu Shuo; Hsieh, Ming Chun; Tung, Kwong Chung; Lin, Tzer Bin.

In: Pain, Vol. 143, No. 1-2, 05.2009, p. 12-20.

Research output: Contribution to journalArticle

Peng, HY, Chen, GD, Lee, SD, Lai, CY, Chiu, CH, Cheng, CL, Chang, YS, Hsieh, MC, Tung, KC & Lin, TB 2009, 'Neuroactive steroids inhibit spinal reflex potentiation by selectively enhancing specific spinal GABAA receptor subtypes', Pain, vol. 143, no. 1-2, pp. 12-20. https://doi.org/10.1016/j.pain.2008.12.023
Peng, Hsien Yu ; Chen, Gin Den ; Lee, Shin Da ; Lai, Cheng Yuan ; Chiu, Chun Hsien ; Cheng, Chen Li ; Chang, Yu Shuo ; Hsieh, Ming Chun ; Tung, Kwong Chung ; Lin, Tzer Bin. / Neuroactive steroids inhibit spinal reflex potentiation by selectively enhancing specific spinal GABAA receptor subtypes. In: Pain. 2009 ; Vol. 143, No. 1-2. pp. 12-20.
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