Neural cell protective compounds isolated from Phoenix hanceana var. formosana

Yi Pei Lin, Tai Yuan Chen, Hsiang Wen Tseng, Mei Hsien Lee, Shui Tein Chen

Research output: Contribution to journalArticle

40 Citations (Scopus)

Abstract

A platform for screening drugs for their ability to protect neuronal cells against cytotoxicity was developed. Nerve growth factor (NGF) differentiates PC12 cells into nerves, and these differentiated PC12 cells enter apoptosis when challenged with 6-hydroxydopamine (6-OHDA). A screening spectrophotometer was used to assay cytotoxicity in these cells; pretreatment with test samples allowed identification of compounds that protected against this neuronal cytotoxicity. The 95% ethanol extract of Phoenix hanceana Naudin var. formosana Beccari. (PH) showed potential neuroprotective activity in these assays. The PH ethanol extract was further fractionated by sequential partitioning with n-hexane, ethyl acetate (EtOAc), n-butanol (n-BuOH), and water. Subsequent rounds of assaying resulted in the isolation of ten constituents, and their structures were characterized by various spectroscopic techniques and identified by comparison with previous data as: isoorientin (1), isovitexin (2), veronicastroside (3), luteolin-7-O-β-d-glucopyranoside (4), isoquercitrin (5), tricin-7-neohesperidoside (6), tricin-7-O-β-d-gluco-pyranoside (7), (+)-catechin (8), (-)-epicatechin (9), and orientin 7-O-β-d-glucopyranoside (10). Among these compounds, isovitexin (2), luteolin-7-O-β-d-glucopyranoside (4) and (+)-catechin (8) showed significant neuroprotective activity in cell viability (WST-8 reduction), anti-apoptosis (Annexin V-FITC/propidium iodide double-labeled flow cytometry), and cellular ROS scavenging assays (besides isovitexin (2)), as well as a decreased caspase-8 activity in 6-OHDA-induced PC12 cells. Hence, isovitexin (2), luteolin-7-O-β-d-glucopyranoside (4), and (+)-catechin (8) protected PC12 cells from 6-OHDA-induced apoptotic neurotoxicity.

Original languageEnglish
Pages (from-to)1173-1181
Number of pages9
JournalPhytochemistry
Volume70
Issue number9
DOIs
Publication statusPublished - Jun 2009

Fingerprint

Catechin
Oxidopamine
PC12 Cells
Luteolin
luteolin
Cytotoxicity
neurons
catechin
cytotoxicity
Assays
neuroprotective effect
homoorientin
Screening
assays
Ethanol
apoptosis
ethanol
cells
isoquercitrin
Apoptosis

Keywords

  • (+)-Catechin
  • 6-Hydroxydopamine
  • Antiapoptosis
  • Cellular ROS scavenging assay
  • Isovitexin
  • Luteolin-7-O-β-d-glucopyranoside
  • Neuroprotective activity
  • Palmae
  • Phoenix hanceana var. formosana

ASJC Scopus subject areas

  • Plant Science
  • Biochemistry
  • Molecular Biology
  • Horticulture

Cite this

Neural cell protective compounds isolated from Phoenix hanceana var. formosana. / Lin, Yi Pei; Chen, Tai Yuan; Tseng, Hsiang Wen; Lee, Mei Hsien; Chen, Shui Tein.

In: Phytochemistry, Vol. 70, No. 9, 06.2009, p. 1173-1181.

Research output: Contribution to journalArticle

Lin, Yi Pei ; Chen, Tai Yuan ; Tseng, Hsiang Wen ; Lee, Mei Hsien ; Chen, Shui Tein. / Neural cell protective compounds isolated from Phoenix hanceana var. formosana. In: Phytochemistry. 2009 ; Vol. 70, No. 9. pp. 1173-1181.
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