Neural cell protective compounds isolated from Phoenix hanceana var. formosana

Yi Pei Lin, Tai Yuan Chen, Hsiang Wen Tseng, Mei Hsien Lee, Shui Tein Chen

Research output: Contribution to journalArticlepeer-review

44 Citations (Scopus)

Abstract

A platform for screening drugs for their ability to protect neuronal cells against cytotoxicity was developed. Nerve growth factor (NGF) differentiates PC12 cells into nerves, and these differentiated PC12 cells enter apoptosis when challenged with 6-hydroxydopamine (6-OHDA). A screening spectrophotometer was used to assay cytotoxicity in these cells; pretreatment with test samples allowed identification of compounds that protected against this neuronal cytotoxicity. The 95% ethanol extract of Phoenix hanceana Naudin var. formosana Beccari. (PH) showed potential neuroprotective activity in these assays. The PH ethanol extract was further fractionated by sequential partitioning with n-hexane, ethyl acetate (EtOAc), n-butanol (n-BuOH), and water. Subsequent rounds of assaying resulted in the isolation of ten constituents, and their structures were characterized by various spectroscopic techniques and identified by comparison with previous data as: isoorientin (1), isovitexin (2), veronicastroside (3), luteolin-7-O-β-d-glucopyranoside (4), isoquercitrin (5), tricin-7-neohesperidoside (6), tricin-7-O-β-d-gluco-pyranoside (7), (+)-catechin (8), (-)-epicatechin (9), and orientin 7-O-β-d-glucopyranoside (10). Among these compounds, isovitexin (2), luteolin-7-O-β-d-glucopyranoside (4) and (+)-catechin (8) showed significant neuroprotective activity in cell viability (WST-8 reduction), anti-apoptosis (Annexin V-FITC/propidium iodide double-labeled flow cytometry), and cellular ROS scavenging assays (besides isovitexin (2)), as well as a decreased caspase-8 activity in 6-OHDA-induced PC12 cells. Hence, isovitexin (2), luteolin-7-O-β-d-glucopyranoside (4), and (+)-catechin (8) protected PC12 cells from 6-OHDA-induced apoptotic neurotoxicity.

Original languageEnglish
Pages (from-to)1173-1181
Number of pages9
JournalPhytochemistry
Volume70
Issue number9
DOIs
Publication statusPublished - Jun 2009

Keywords

  • (+)-Catechin
  • 6-Hydroxydopamine
  • Antiapoptosis
  • Cellular ROS scavenging assay
  • Isovitexin
  • Luteolin-7-O-β-d-glucopyranoside
  • Neuroprotective activity
  • Palmae
  • Phoenix hanceana var. formosana

ASJC Scopus subject areas

  • Plant Science
  • Biochemistry
  • Molecular Biology
  • Horticulture

Fingerprint Dive into the research topics of 'Neural cell protective compounds isolated from Phoenix hanceana var. formosana'. Together they form a unique fingerprint.

Cite this