Neisseria conserved protein DMP19 is a DNA mimic protein that prevents DNA binding to a hypothetical nitrogen-response transcription factor

Hao Ching Wang, Tzu Ping Ko, Mao Lun Wu, Shan Chi Ku, Hsing Ju Wu, Andrew H J Wang

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

DNA mimic proteins occupy the DNA binding sites of DNA-binding proteins, and prevent these sites from being accessed by DNA. We show here that the Neisseria conserved hypothetical protein DMP19 acts as a DNA mimic. The crystal structure of DMP19 shows a dsDNA-like negative charge distribution on the surface, suggesting that this protein should be added to the short list of known DNA mimic proteins. The crystal structure of another related protein, NHTF (Neisseria hypothetical transcription factor), provides evidence that it is a member of the xenobiotic-response element (XRE) family of transcriptional factors. NHTF binds to a palindromic DNA sequence containing a 5′-TGTNAN11TNACA-3′ recognition box that controls the expression of an NHTF-related operon in which the conserved nitrogen-response protein [i.e. (Protein-PII) uridylyltransferase] is encoded. The complementary surface charges between DMP19 and NHTF suggest specific charge-charge interaction. In a DNA-binding assay, we found that DMP19 can prevent NHTF from binding to its DNA-binding sites. Finally, we used an in situ gene regulation assay to provide evidence that NHTF is a repressor of its down-stream genes and that DMP19 can neutralize this effect. We therefore conclude that the interaction of DMP19 and NHTF provides a novel gene regulation mechanism in Neisseria spps.

Original languageEnglish
Pages (from-to)5718-5730
Number of pages13
JournalNucleic Acids Research
Volume40
Issue number12
DOIs
Publication statusPublished - Jul 2012
Externally publishedYes

Fingerprint

Neisseria
DNA-Binding Proteins
Transcription Factors
Nitrogen
DNA
Proteins
regulatory protein uridylyltransferase
Binding Sites
Genes
Response Elements
Xenobiotics
Operon
Membrane Proteins

ASJC Scopus subject areas

  • Genetics

Cite this

Neisseria conserved protein DMP19 is a DNA mimic protein that prevents DNA binding to a hypothetical nitrogen-response transcription factor. / Wang, Hao Ching; Ko, Tzu Ping; Wu, Mao Lun; Ku, Shan Chi; Wu, Hsing Ju; Wang, Andrew H J.

In: Nucleic Acids Research, Vol. 40, No. 12, 07.2012, p. 5718-5730.

Research output: Contribution to journalArticle

Wang, Hao Ching ; Ko, Tzu Ping ; Wu, Mao Lun ; Ku, Shan Chi ; Wu, Hsing Ju ; Wang, Andrew H J. / Neisseria conserved protein DMP19 is a DNA mimic protein that prevents DNA binding to a hypothetical nitrogen-response transcription factor. In: Nucleic Acids Research. 2012 ; Vol. 40, No. 12. pp. 5718-5730.
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