Neisseria conserved hypothetical protein DMP12 is a DNA mimic that binds to histone-like HU protein

Hao Ching Wang, Mao Lun Wu, Tzu Ping Ko, Andrew H J Wang

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

DNA mimic proteins are unique factors that control the DNA-binding activity of target proteins by directly occupying their DNA-binding sites. To date, only a few DNA mimic proteins have been reported and their functions analyzed. Here, we present evidence that the Neisseria conserved hypothetical protein DMP12 should be added to this list. Our gel filtration and analytical ultracentrifugation results showed that the DMP12 monomer interacts with the dimeric form of the bacterial histone-like protein HU. Subsequent structural analysis of DMP12 showed that the shape and electrostatic surface of the DMP12 monomer are similar to those of the straight portion of the bent HU-bound DNA and complementary to those of HU protein dimer. DMP12 also protects HU protein from limited digestion by trypsin and enhances the growth rate Escherichia coli. Functionally, HU proteins participate in bacterial nucleoid formation, as well as recombination, gene regulation and DNA replication. The interaction between DMP12 and HU protein might, therefore, play important roles in these DNA-related mechanisms.

Original languageEnglish
Pages (from-to)5127-5138
Number of pages12
JournalNucleic Acids Research
Volume41
Issue number9
DOIs
Publication statusPublished - May 2013
Externally publishedYes

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Neisseria
Histones
DNA
Proteins
Ultracentrifugation
Static Electricity
DNA Replication
Trypsin
Genetic Recombination
Proteolysis
Gel Chromatography
Complementary DNA
Binding Sites
Escherichia coli

ASJC Scopus subject areas

  • Genetics

Cite this

Neisseria conserved hypothetical protein DMP12 is a DNA mimic that binds to histone-like HU protein. / Wang, Hao Ching; Wu, Mao Lun; Ko, Tzu Ping; Wang, Andrew H J.

In: Nucleic Acids Research, Vol. 41, No. 9, 05.2013, p. 5127-5138.

Research output: Contribution to journalArticle

Wang, Hao Ching ; Wu, Mao Lun ; Ko, Tzu Ping ; Wang, Andrew H J. / Neisseria conserved hypothetical protein DMP12 is a DNA mimic that binds to histone-like HU protein. In: Nucleic Acids Research. 2013 ; Vol. 41, No. 9. pp. 5127-5138.
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