Nebulin cDNAs detect a 25-kilobase transcript in skeletal muscle and localize to human chromosome 2

Hansell Stedman, Karen Browning, Noelynn Oliver, Marion Oronzi-Scott, Kenneth Fischbeck, Satyapriya Sarkar, James Sylvester, Roy Schmickel, Kuan Wang

Research output: Contribution to journalArticle

49 Citations (Scopus)

Abstract

By virtue of the protein's size, myofibrillar localization, and proposed functional role, the gene encoding the giant sarcomere matrix protein nebulin represents a possible site for myopathic mutations. Using polyclonal anti-nebulin antisera to screen a cDNA expression library, we have isolated and characterized two separate human fetal muscle cDNA clones. By recovering fusion polypeptide-bound portions of our polyclonal antiserum and reutilizing them to probe Western blots, we further demonstrate that the expressed cDNAs encode polypeptide epitopes unique to the protein nebulin. Both cDNAs detect a 25-kb skeletal muscle RNA transcript and localize to human chromosome 2. The identification of nebulin cDNA clones enables the complete analysis of this enormous mRNA by transcript walking through muscle cDNA libraries. Here we report a restriction map of the 3′ end of the human nebulin transcript, with reference to the genomic fragments identified by the cDNA.

Original languageEnglish
Pages (from-to)1-7
Number of pages7
JournalGenomics
Volume2
Issue number1
DOIs
Publication statusPublished - Jan 1988
Externally publishedYes

ASJC Scopus subject areas

  • Genetics

Fingerprint Dive into the research topics of 'Nebulin cDNAs detect a 25-kilobase transcript in skeletal muscle and localize to human chromosome 2'. Together they form a unique fingerprint.

  • Cite this

    Stedman, H., Browning, K., Oliver, N., Oronzi-Scott, M., Fischbeck, K., Sarkar, S., Sylvester, J., Schmickel, R., & Wang, K. (1988). Nebulin cDNAs detect a 25-kilobase transcript in skeletal muscle and localize to human chromosome 2. Genomics, 2(1), 1-7. https://doi.org/10.1016/0888-7543(88)90102-4