Nebulin as a giant actin-binding template protein in skeletal muscle sarcomere Interaction of actin and cloned human nebulin fragments

Jian Ping Jin, Kuan Wang

Research output: Contribution to journalArticle

66 Citations (Scopus)

Abstract

Nebulin is a family of giant sarcomere matrix proteins of 6OO-900 kDa in most vertebrate skeletal muscles. Recent sequence analysis suggests that human nebulin is mainly composed of a large number (> 200) of conserved repeats of ∼ 35 residues. Two cloned nebulin fragments, consisting of 6 and 8 of the repeats, have been expressed in E. coli using the pET3d vector. Both F-actin cosedimentation and solid-phase binding assays demonstrated a specific binding of these nebulin fragments to actin. This finding suggests that nebulin is a giant protein which binds actin at multiple sites in a template-manner. The presence of an actin-binding template protein in the skeletal muscle sarcomere may have significant implications in the assembly and function of the contractile apparatus.

Original languageEnglish
Pages (from-to)93-96
Number of pages4
JournalFEBS Letters
Volume281
Issue number1-2
DOIs
Publication statusPublished - Apr 9 1991
Externally publishedYes

Keywords

  • Actin-binding protein
  • cDNA expression in E. Coli
  • ELISA
  • Muscle thin filament
  • Nebulin

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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