Naturally occurring chlorophyll derivatives inhibit aflatoxin B1-DNA adduct formation in hepatoma cells

Ching Yun Hsu, Yue-Hwa Chen, Pi Yu Chao, Chiao-Ming Chen, Ling Ling Hsieh, Xue-Ping Hu

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

The inhibitory effects of four chlorophyll derivatives (chlorophyllide [Chlide] a and b and pheophorbide [Pho] a and b) on aflatoxin B1 (AFB1)-DNA adduct formation, and on the modulation of hepatic glutathione S-transferase (GST) were evaluated in murine hepatoma (Hepa-1) cells. Enzyme-linked immunosorbent assay showed that pretreatment with Chlide or Pho significantly reduced the formation of AFB1-DNA adducts, and that Pho was the most potent inhibitor. However, wash-out prior to adding AFB1 totally eliminated inhibition by Childe and partially eliminated inhibition by Pho, indicating that the inhibitory effect of Chlide, and to some extent Pho, was mediated through direct trapping of AFB1. Furthermore, spectrophotometric analysis showed that Pho treatment could increase GST activity in Hepa-1 cells. These observations indicate that the chlorophyll derivatives studied may attenuate AFB1-induced DNA damage in the Hepa-1 cell by direct trapping of AFB1. Pho provided additional protection not only by direct trapping, but also by increasing GST activity against hepatic AFB1 metabolites.

Original languageEnglish
Pages (from-to)98-104
Number of pages7
JournalMutation Research - Genetic Toxicology and Environmental Mutagenesis
Volume657
Issue number2
DOIs
Publication statusPublished - Dec 8 2008

Fingerprint

Aflatoxin B1
Chlorophyll
Hepatocellular Carcinoma
Chlorophyllides
Glutathione Transferase
Liver
DNA Damage
Enzyme-Linked Immunosorbent Assay
aflatoxin B1-DNA adduct

Keywords

  • Aflatoxin B
  • Chlorophyll derivatives
  • Chlorophyllide
  • Glutathione S-transferase
  • GST
  • Pheophorbide

ASJC Scopus subject areas

  • Health, Toxicology and Mutagenesis
  • Genetics

Cite this

Naturally occurring chlorophyll derivatives inhibit aflatoxin B1-DNA adduct formation in hepatoma cells. / Hsu, Ching Yun; Chen, Yue-Hwa; Chao, Pi Yu; Chen, Chiao-Ming; Hsieh, Ling Ling; Hu, Xue-Ping.

In: Mutation Research - Genetic Toxicology and Environmental Mutagenesis, Vol. 657, No. 2, 08.12.2008, p. 98-104.

Research output: Contribution to journalArticle

Hsu, Ching Yun ; Chen, Yue-Hwa ; Chao, Pi Yu ; Chen, Chiao-Ming ; Hsieh, Ling Ling ; Hu, Xue-Ping. / Naturally occurring chlorophyll derivatives inhibit aflatoxin B1-DNA adduct formation in hepatoma cells. In: Mutation Research - Genetic Toxicology and Environmental Mutagenesis. 2008 ; Vol. 657, No. 2. pp. 98-104.
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