Nasal carriage of methicillin-resistant Staphylococcus aureus among pediatricians in Taiwan

Yhu Chering Huang, Lin Hui Su, Tzou Yien Lin

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Background: Health care workers (HCWs) are at the interface between hospitals and communities. The survey for methicillin-resistant Staphylococcus aureus (MRSA) carriage among HCWs has mostly been conducted to investigate outbreaks or endemics. Community-associated MRSA are prevalent among children in Taiwan. We conducted this study to better understand the carriage rate of MRSA among pediatricians in non-outbreak situations in Taiwan,. Methods: A total of 220 pediatricians from Taiwan who attended the annual meeting of Taiwan Pediatric Association in April, 2010 were recruited to participate in this study and were sampled from the nares for the detection of MRSA by polymerase chain reaction (PCR) and further by culture. The following molecular analyses were performed, including pulsed-field gel electrophoresis (PFGE), multilocus sequence typing (MLST), typing of staphylococcal cassette chromosome mec (SCCmec) and the presence of Panton-Valentine leukocidin (PVL) genes. Results: MRSA was detected from 15 attendees (6.8%) by PCR. MRSA-colonized attendees had a significantly lower rate (0.041) of working in the medical center, while borderline significantly higher rate of working in the Regional Hospital (p=0.056), than those without MRSA colonization. From those 15 samples, 12 MRSA isolates were identified by culture and molecularly characterized. Three PFGE patterns, two sequence types (ST 59, ST 508), and two SCC mec types (IV and VT) were identified, respectively. Five isolates, including three carrying SCCmec types VT, were PVL-positive. All 12 isolates were susceptible to vancomycin, teicoplanin, linezolid, fusidic acid, trimethoprim/sulfamethoxazole, and doxycyclin, and resistant to penicillin. Conclusion/significance: Around seven percent of pediatricians in Taiwan harbored CA-MRSA in their nares.

Original languageEnglish
Article numbere82472
JournalPLoS One
Volume8
Issue number11
DOIs
Publication statusPublished - Nov 26 2013
Externally publishedYes

Fingerprint

pediatricians
Methicillin
Methicillin-Resistant Staphylococcus aureus
Taiwan
Nose
Linezolid
Pulsed Field Gel Electrophoresis
Polymerase chain reaction
pulsed-field gel electrophoresis
health care workers
Chromosomes
Electrophoresis
Health care
Gels
polymerase chain reaction
Fusidic Acid
chromosomes
Delivery of Health Care
Pediatricians
methicillin-resistant Staphylococcus aureus

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Nasal carriage of methicillin-resistant Staphylococcus aureus among pediatricians in Taiwan. / Huang, Yhu Chering; Su, Lin Hui; Lin, Tzou Yien.

In: PLoS One, Vol. 8, No. 11, e82472, 26.11.2013.

Research output: Contribution to journalArticle

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abstract = "Background: Health care workers (HCWs) are at the interface between hospitals and communities. The survey for methicillin-resistant Staphylococcus aureus (MRSA) carriage among HCWs has mostly been conducted to investigate outbreaks or endemics. Community-associated MRSA are prevalent among children in Taiwan. We conducted this study to better understand the carriage rate of MRSA among pediatricians in non-outbreak situations in Taiwan,. Methods: A total of 220 pediatricians from Taiwan who attended the annual meeting of Taiwan Pediatric Association in April, 2010 were recruited to participate in this study and were sampled from the nares for the detection of MRSA by polymerase chain reaction (PCR) and further by culture. The following molecular analyses were performed, including pulsed-field gel electrophoresis (PFGE), multilocus sequence typing (MLST), typing of staphylococcal cassette chromosome mec (SCCmec) and the presence of Panton-Valentine leukocidin (PVL) genes. Results: MRSA was detected from 15 attendees (6.8{\%}) by PCR. MRSA-colonized attendees had a significantly lower rate (0.041) of working in the medical center, while borderline significantly higher rate of working in the Regional Hospital (p=0.056), than those without MRSA colonization. From those 15 samples, 12 MRSA isolates were identified by culture and molecularly characterized. Three PFGE patterns, two sequence types (ST 59, ST 508), and two SCC mec types (IV and VT) were identified, respectively. Five isolates, including three carrying SCCmec types VT, were PVL-positive. All 12 isolates were susceptible to vancomycin, teicoplanin, linezolid, fusidic acid, trimethoprim/sulfamethoxazole, and doxycyclin, and resistant to penicillin. Conclusion/significance: Around seven percent of pediatricians in Taiwan harbored CA-MRSA in their nares.",
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