Nanowire field effect transistor with its sub-picomolar label-free biosensing capability toward a gene mutation

Chi Chang Wu; Fu Hsiang Ko; Ting Siang Su; Bo Syuan Li; Wen Fa Wu

doi:10.1109/INEC.2010.5425155

Nanowire field effect transistor with its sub-picomolar label-free biosensing capability toward a gene mutation

Chi Chang Wu, Fu Hsiang Ko, Ting Siang Su, Bo Syuan Li, Wen Fa Wu

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

1 Citation (Scopus)

Abstract

We describe the efficiency of deoxyribonucleic acid (DNA) immobilization onto a nanowire (NW) after employing various surface cleaning methods. From surface tension measurements and fluorescence microscopy images of the silicon oxide surfaces, we determined that the effectiveness of surface cleaning using an acetone/ethanol mixture was similar to that of piranha solution (sulfuric acid/hydrogen peroxide). Thus, we employed surface cleaning with an acetone/ethanol mixture en route toward the fabrication of a series of label-free, back-gated, 60-nm nanowire field-effect transistor (NWFET) sensors for the detection of the BRAFV599E oncogene. We applied the NWFET sensor to the successful detection of the hybridization and dehybridization processes of the BRAFV599E mutation gene.

Original language	English
Title of host publication	INEC 2010 - 2010 3rd International Nanoelectronics Conference, Proceedings
Pages	872-873
Number of pages	2
DOIs	https://doi.org/10.1109/INEC.2010.5425155
Publication status	Published - 2010
Externally published	Yes
Event	2010 3rd International Nanoelectronics Conference, INEC 2010 - Hongkong, China Duration: Jan 3 2010 → Jan 8 2010

Other

Other	2010 3rd International Nanoelectronics Conference, INEC 2010
Country/Territory	China
City	Hongkong
Period	1/3/10 → 1/8/10

ASJC Scopus subject areas

Electrical and Electronic Engineering

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10.1109/INEC.2010.5425155

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Nanowire field effect transistor with its sub-picomolar label-free biosensing capability toward a gene mutation. / Wu, Chi Chang; Ko, Fu Hsiang; Su, Ting Siang; Li, Bo Syuan; Wu, Wen Fa.

INEC 2010 - 2010 3rd International Nanoelectronics Conference, Proceedings. 2010. p. 872-873 5425155.

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Wu, CC, Ko, FH, Su, TS, Li, BS & Wu, WF 2010, Nanowire field effect transistor with its sub-picomolar label-free biosensing capability toward a gene mutation. in INEC 2010 - 2010 3rd International Nanoelectronics Conference, Proceedings., 5425155, pp. 872-873, 2010 3rd International Nanoelectronics Conference, INEC 2010, Hongkong, China, 1/3/10. https://doi.org/10.1109/INEC.2010.5425155

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title = "Nanowire field effect transistor with its sub-picomolar label-free biosensing capability toward a gene mutation",

abstract = "We describe the efficiency of deoxyribonucleic acid (DNA) immobilization onto a nanowire (NW) after employing various surface cleaning methods. From surface tension measurements and fluorescence microscopy images of the silicon oxide surfaces, we determined that the effectiveness of surface cleaning using an acetone/ethanol mixture was similar to that of piranha solution (sulfuric acid/hydrogen peroxide). Thus, we employed surface cleaning with an acetone/ethanol mixture en route toward the fabrication of a series of label-free, back-gated, 60-nm nanowire field-effect transistor (NWFET) sensors for the detection of the BRAFV599E oncogene. We applied the NWFET sensor to the successful detection of the hybridization and dehybridization processes of the BRAFV599E mutation gene.",

author = "Wu, {Chi Chang} and Ko, {Fu Hsiang} and Su, {Ting Siang} and Li, {Bo Syuan} and Wu, {Wen Fa}",

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AU - Wu, Chi Chang

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AU - Su, Ting Siang

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AU - Wu, Wen Fa

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N2 - We describe the efficiency of deoxyribonucleic acid (DNA) immobilization onto a nanowire (NW) after employing various surface cleaning methods. From surface tension measurements and fluorescence microscopy images of the silicon oxide surfaces, we determined that the effectiveness of surface cleaning using an acetone/ethanol mixture was similar to that of piranha solution (sulfuric acid/hydrogen peroxide). Thus, we employed surface cleaning with an acetone/ethanol mixture en route toward the fabrication of a series of label-free, back-gated, 60-nm nanowire field-effect transistor (NWFET) sensors for the detection of the BRAFV599E oncogene. We applied the NWFET sensor to the successful detection of the hybridization and dehybridization processes of the BRAFV599E mutation gene.

AB - We describe the efficiency of deoxyribonucleic acid (DNA) immobilization onto a nanowire (NW) after employing various surface cleaning methods. From surface tension measurements and fluorescence microscopy images of the silicon oxide surfaces, we determined that the effectiveness of surface cleaning using an acetone/ethanol mixture was similar to that of piranha solution (sulfuric acid/hydrogen peroxide). Thus, we employed surface cleaning with an acetone/ethanol mixture en route toward the fabrication of a series of label-free, back-gated, 60-nm nanowire field-effect transistor (NWFET) sensors for the detection of the BRAFV599E oncogene. We applied the NWFET sensor to the successful detection of the hybridization and dehybridization processes of the BRAFV599E mutation gene.

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BT - INEC 2010 - 2010 3rd International Nanoelectronics Conference, Proceedings

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Nanowire field effect transistor with its sub-picomolar label-free biosensing capability toward a gene mutation

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