Nanostructured titanium dioxide layer combined with reactive functional groups as a promising biofunctional surface for biomedical applications

Chi Ming Wu, Chung Ming Liu, Keng Liang Ou, Hsi Jen Chiang, Erwan Sugiatno, Chia Hung Wu, Hsiu Ju Yen, Hsin Hua Chou

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

In the present study, low-temperature plasma combined with allylamine polymerization approach was used to coat amino-groups on oxide layer of plasma-oxidized biomedical titanium (Ti) for protein immobilization. Scanning electron microscopy, X-ray diffractometer, transmission electron microscopy, X-ray photoemission spectroscopy, secondary ion mass spectrometer and 2, 4, 6-trinitrobenzenesulfonic acid assay were utilized to investigate the surface and microstructural properties of the plasma-oxidized, plasma-polymerized and protein-immobilized samples. Analytical results indicated that the presence of a nanostructured rutile-TiO 2 thin layer could be found on the plasma-oxidized samples. The thickness of nanostructured rutile-TiO 2 layer increased with increasing plasma treatment power and period. As the plasma-oxidized samples underwent plasma polymerization with allylamine, amino-groups (NH 2 ) were uniformly coated on nanostructured rutile-TiO 2 layer. It was also found that Ti surface with thick oxide layer exhibited higher amounts of amino-groups deposition. After protein immobilization, the plasma-polymerized samples presented a formation of uniform streak-like immobilized protein clusters. Therefore, biomedical Ti with nanostructured rutile-TiO 2 layer is a promising biomaterial that can be applied to cross-link with other biomolecules for promoting the bone healing and regeneration.

Original languageEnglish
JournalCeramics International
DOIs
Publication statusPublished - Jan 1 2019

Fingerprint

Titanium dioxide
Functional groups
Plasmas
Titanium
Allylamine
Immobilized Proteins
Proteins
Oxides
Trinitrobenzenesulfonic Acid
Plasma polymerization
titanium dioxide
Diffractometers
Biocompatible Materials
Biomolecules
Mass spectrometers
Photoelectron spectroscopy
X ray spectroscopy
Biomaterials
Assays
Bone

Keywords

  • Allylamine polymerization
  • Low-temperature plasma
  • Oxidation
  • Protein immobilization

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Ceramics and Composites
  • Process Chemistry and Technology
  • Surfaces, Coatings and Films
  • Materials Chemistry

Cite this

Nanostructured titanium dioxide layer combined with reactive functional groups as a promising biofunctional surface for biomedical applications. / Wu, Chi Ming; Liu, Chung Ming; Ou, Keng Liang; Chiang, Hsi Jen; Sugiatno, Erwan; Wu, Chia Hung; Yen, Hsiu Ju; Chou, Hsin Hua.

In: Ceramics International, 01.01.2019.

Research output: Contribution to journalArticle

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