Nanoplasmonic structure of a polycarbonate substrate integrated with parallel microchannels for label-free multiplex detection

Yi Jung Lu, Han Yun Hsieh, Wen Chang Kuo, Pei Kuen Wei, Horn Jiunn Sheen, Hidetoshi Tahara, Te Wei Chiu, Yu Jui Fan

Research output: Contribution to journalArticlepeer-review

Abstract

In this study, a multiplex detection system was proposed by integrating a localized surface plasmon resonance (LSPR) sensing array and parallel microfluidic channels. The LSPR sensing array was fabricated by nanoimprinting and gold sputter on a polycarbonate (PC) substrate. The polydimethylsiloxane (PDMS) microfluidic channels and PC LSPR sensing array were bound together through (3-aminopropyl)triethoxysilane (APTES) surface treatment and oxygen plasma treatment. The resonant spectrum of the LSPR sensing device was obtained by broadband white-light illumi-nation and polarized wavelength measurements with a spectrometer. The sensitivity of the LSPR sensing device was measured using various ratios of glycerol to water solutions with different refractive indices. Multiplex detection was demonstrated using human immunoglobulin G (IgG), IgA, and IgM. The anti-IgG, anti-IgA, and anti-IgM were separately modified in each sensing region. Various concentrations of human IgG, IgA, and IgM were prepared to prove the concept that the parallel sensing device can be used to detect different targets.

Original languageEnglish
Article number3294
JournalPolymers
Volume13
Issue number19
DOIs
Publication statusPublished - Oct 1 2021

Keywords

  • Biosensor
  • Multiplex detection
  • Nanoimprinting
  • Surface plasmon resonance (SPR)

ASJC Scopus subject areas

  • Chemistry(all)
  • Polymers and Plastics

Fingerprint

Dive into the research topics of 'Nanoplasmonic structure of a polycarbonate substrate integrated with parallel microchannels for label-free multiplex detection'. Together they form a unique fingerprint.

Cite this