Nanofiltration, a new specific virus elimination method applied to high-purity factor IX and factor XI concentrates

M. Burnouf-Radosevich, P. Appourchaux, J. J. Huart, T. Burnouf

Research output: Contribution to journalArticle

100 Citations (Scopus)

Abstract

We have validated the use of two new regenerate multilayered structured cellulose membranes (BMM), Planova 15 N and Planova 35 N, with respective mean pore sizes of 15 and 35 nm, as a new filtration system to eliminate viruses in highly purified factor IX and factor XI concentrates. Virus spiking experiments indicated that single dead-end filtration on the membranes could remove more than 5.7-7.8 log10 of human immunodeficiency virus, bovine viral diarrhoea virus, porcine pseudorabies virus, reovirus type 3, and simian virus 40, as wall as the small non-enveloped viruses, poliovirus Sabin type 1 and bovine parvovirus. In vitro control tests and animal studies (Wessler stasis model, rat hypotension model) of the two concentrates did not reveal any significant differences with the non-nanofiltered material. Viral filtration of plasma derivatives on porous polymeric membranes might be an essential step in the improvement of their viral safety.

Original languageEnglish
Pages (from-to)132-138
Number of pages7
JournalVox Sanguinis
Volume67
Issue number2
Publication statusPublished - 1994
Externally publishedYes

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Factor XI
Factor IX
Viruses
Membranes
Bocavirus
Mammalian orthoreovirus 3
Bovine Viral Diarrhea Viruses
Suid Herpesvirus 1
Simian virus 40
Poliovirus
Cellulose
Hypotension
Swine
HIV
Safety

ASJC Scopus subject areas

  • Hematology

Cite this

Nanofiltration, a new specific virus elimination method applied to high-purity factor IX and factor XI concentrates. / Burnouf-Radosevich, M.; Appourchaux, P.; Huart, J. J.; Burnouf, T.

In: Vox Sanguinis, Vol. 67, No. 2, 1994, p. 132-138.

Research output: Contribution to journalArticle

Burnouf-Radosevich, M. ; Appourchaux, P. ; Huart, J. J. ; Burnouf, T. / Nanofiltration, a new specific virus elimination method applied to high-purity factor IX and factor XI concentrates. In: Vox Sanguinis. 1994 ; Vol. 67, No. 2. pp. 132-138.
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