NanoBioAnalytical characterization of extracellular vesicles in 75-nm nanofiltered human plasma for transfusion: A tool to improve transfusion safety

Sameh Obeid, Pei Shan Sung, Benoit Le Roy, Ming Li Chou, Shie Liang Hsieh, Celine Elie-Caille, Thierry Burnouf, Wilfrid Boireau

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Plasma transfusion induces some transfusion related acute lung injury (TRALI) mediated through neutrophil extracellular traps (NETs). We investigated whether extracellular vesicles (EVs) present in plasma or obtained from resting (N-PEVs) or thrombin activated platelets (T-PEVs) can trigger NETs, and whether 75 nm-nanofiltration, to partially remove EVs, prohibits NETs formation. EVs size and concentration were determined by conventional biophysical approaches and by an original NanoBioAnalytical (NBA) platform based on EV immunocapture biochip, combining Surface Plasmon Resonance Imaging (SPRi) and Atomic Force Microscopy (AFM) exploration. EVs effective diameter was in the 25-1000 nm range, with a majority (≈ 90%) ≤ 100 nm. Both T-PEVs in buffer (but not N-PEVs) and non-nanofiltered plasma containing T-PEVs triggered NETs formation. Nanofiltration depleted large EVs (> 70 nm) and decreased NETs formation. The NBA platform was found to be a suitable tool to investigate the safety of plasma for transfusion.

Original languageEnglish
Article number101977
Pages (from-to)101977
JournalNanomedicine: Nanotechnology, Biology, and Medicine
Volume20
DOIs
Publication statusPublished - Aug 1 2019

Fingerprint

Plasma (human)
Safety
Plasmas
Nanofiltration
Biochips
Surface plasmon resonance
Platelets
Surface Plasmon Resonance
Thrombin
Acute Lung Injury
Atomic Force Microscopy
Atomic force microscopy
Buffers
Plug-in electric vehicles
Extracellular Vesicles
Blood Platelets
Imaging techniques
Extracellular Traps

Keywords

  • AFM
  • Extra-cellular vesicles (EV)
  • NanoBioAnalytical platform
  • Nanofiltration
  • NETs
  • Plasma
  • Platelets
  • SPRi

ASJC Scopus subject areas

  • Bioengineering
  • Medicine (miscellaneous)
  • Molecular Medicine
  • Biomedical Engineering
  • Materials Science(all)
  • Pharmaceutical Science

Cite this

NanoBioAnalytical characterization of extracellular vesicles in 75-nm nanofiltered human plasma for transfusion : A tool to improve transfusion safety. / Obeid, Sameh; Sung, Pei Shan; Le Roy, Benoit; Chou, Ming Li; Hsieh, Shie Liang; Elie-Caille, Celine; Burnouf, Thierry; Boireau, Wilfrid.

In: Nanomedicine: Nanotechnology, Biology, and Medicine, Vol. 20, 101977, 01.08.2019, p. 101977.

Research output: Contribution to journalArticle

Obeid, Sameh ; Sung, Pei Shan ; Le Roy, Benoit ; Chou, Ming Li ; Hsieh, Shie Liang ; Elie-Caille, Celine ; Burnouf, Thierry ; Boireau, Wilfrid. / NanoBioAnalytical characterization of extracellular vesicles in 75-nm nanofiltered human plasma for transfusion : A tool to improve transfusion safety. In: Nanomedicine: Nanotechnology, Biology, and Medicine. 2019 ; Vol. 20. pp. 101977.
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