N-nitroso-2-acetylaminofluorene: A direct-acting carcinogen inducing hepatocellular carcinoma in Sprague-Dawley rats

Y. S. Ho, J. K. Lin

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4 Citations (Scopus)


To compare the hepatotoxicity and hepatocarcinogenicity of N-nitroso-2-acetylaminofluorene (NO-AAF) and its parent compound, 2-acetylaminofluorene (AAF), male Sprague-Dawley rats were given intraperitoneal (i.p.) or subcutaneous (s.c.) injections of AAF or NO-AAF (60 mg/kg body weight/week) for ten months. In the AAF group, morphological changes were produced which involved gross distortions of the liver with multiple nodule formations. The rat livers in the NO-AAF group appeared to be smooth with a blunt-thick superior segment of the lateral lobe. The serum γ-glutamyl transpeptidase activity in both the AAF group and the NO-AAF group was significantly elevated (P <0.0005). The present study shows that i.p. and s.c. injections of NO-AAF resulted in a high incidence of well-differentiated hepatocellular carcinomas (HCC) (7/9 and 4/6, respectively), while poorly differentiated HCCs were induced by i.p. or s.c. administration of AAF (6/9 or 2/6, respectively). Subcutaneous lesions consisting of an inflammatory reaction and fibroadenoma formation were observed in the NO-AAF-treated rats, whereas no such skin lesions were detected in the AAF-treated animals. These results suggest that NO-AAF is a new direct-acting carcinogen which may be useful for investigating hepatocarcinogenesis.

Original languageEnglish
Pages (from-to)794-800
Number of pages7
JournalJapanese Journal of Cancer Research
Issue number8
Publication statusPublished - 1994
Externally publishedYes



  • γ-glutamyl transpeptidase
  • AAF
  • Fibroadenoma
  • Hepatocarcinogenicity
  • NO-AAF

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

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