N-linked glycosylation of hepatitis B surface antigens is involved but not essential in the assembly of hepatitis delta virus

Chuan Jen Wang, Shian Ying Sung, Ding Shinn Chen, Pei Jer Chen

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Hepatitis delta virus (HDV) is a defective virus requiring the hepatitis B virus (HBV) to provide hepatitis B surface antigens as the envelope protein. The hepatitis B surface antigens are posttranslationally modified by N-linked glycosylation, and its significance in HDV assembly was investigated with a cotransfection system using human hepatoma cell line Huh-7. After the N-linked glycosylation of HBsAg was blocked by tunicamycin treatment, the packaging of HDV in the culture system could be suppressed to a level as low as 5-10% of the untreated control. The extent of inhibition correlated with the increased concentrations of tunicamycin. In contrast, the loss of HBsAg glycosylation did not affect the efficiency of assembly of HBV particles. When the N-linked glycosylation site of small HBsAg at amino acid 146 was mutated from asparagine to glutamine, the mutant HBsAg packaged only a modest amount of HDV particles. The quantity and kinetics of formation of HDV particles in culture system were reduced by the depletion of HBsAg glycosylation. Therefore HDV, similar to influenza and vesicular stomatitis viruses, depends on glycosylation of the envelope proteins as a signal for envelope protein maturation and for virion formation.

Original languageEnglish
Pages (from-to)28-36
Number of pages9
JournalVirology
Volume220
Issue number1
DOIs
Publication statusPublished - Jun 1 1996
Externally publishedYes

Fingerprint

Hepatitis Delta Virus
Hepatitis B Surface Antigens
Glycosylation
Virion
Tunicamycin
Hepatitis B virus
Defective Viruses
Virus Assembly
Vesicular Stomatitis
Asparagine
Product Packaging
Glutamine
Human Influenza
Hepatocellular Carcinoma
Proteins
Viruses
Amino Acids
Cell Line

ASJC Scopus subject areas

  • Medicine(all)
  • Virology

Cite this

N-linked glycosylation of hepatitis B surface antigens is involved but not essential in the assembly of hepatitis delta virus. / Wang, Chuan Jen; Sung, Shian Ying; Chen, Ding Shinn; Chen, Pei Jer.

In: Virology, Vol. 220, No. 1, 01.06.1996, p. 28-36.

Research output: Contribution to journalArticle

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