N-Arachidonyl dopamine sensitizes rat capsaicin-sensitive lung vagal afferents via activation of TRPV1 receptors

Chun-Chun Hsu, Mauo-Ying Bien, Ya-Tien Huang, Ting Ruan, Yu-Ru Kou, You-Shuei Lin

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

We investigated the effect of N-arachidonyl dopamine (NADA), an endogenous agonist of both transient receptor potential vanilloid 1 (TRPV1) and cannabinoid CB1 receptors, on the sensitivity of rat capsaicin-sensitive lung vagal afferent (CSLVA) fibers. In artificially ventilated rats, an intravenous infusion of NADA (400 μg/kg/ml, 0.5 ml/min for 2 min) mildly elevated the baseline CSLVA fiber activity, whereas it markedly potentiated CSLVA fiber responses to a right atrial injection of capsaicin or adenosine, and to lung inflation. The potentiating effect on CSLVA fiber sensitivity to an adenosine injection or lung inflation was blocked by capsazepine pretreatment (a TRPV1 receptor antagonist), but was unaffected by AM251 pretreatment (a CB1 receptor antagonist). In spontaneously breathing rats, a NADA infusion similarly potentiated the CSLVA fiber-mediated apneic response evoked by an adenosine injection, and this potentiating effect was also prevented by capsazepine pretreatment. We concluded that NADA at the dose tested non-specifically increases CSLVA fiber sensitivity to chemical and mechanical stimulation via activation of TRPV1 receptors.

Original languageEnglish
Pages (from-to)323-332
Number of pages10
JournalRespiratory Physiology and Neurobiology
Volume167
Issue number3
DOIs
Publication statusPublished - Jul 31 2009

Fingerprint

Capsaicin
Lung
Adenosine
Cannabinoid Receptor CB1
Economic Inflation
Injections
Chemical Stimulation
vanilloid receptor subtype 1
arachidonyl dopamine
Dopamine Agonists
Intravenous Infusions
Respiration

Keywords

  • Afferent sensitization
  • Lung
  • Lung vagal C fibers
  • NADA
  • TRPV1 receptors

ASJC Scopus subject areas

  • Physiology
  • Pulmonary and Respiratory Medicine
  • Neuroscience(all)

Cite this

N-Arachidonyl dopamine sensitizes rat capsaicin-sensitive lung vagal afferents via activation of TRPV1 receptors. / Hsu, Chun-Chun; Bien, Mauo-Ying; Huang, Ya-Tien; Ruan, Ting; Kou, Yu-Ru; Lin, You-Shuei.

In: Respiratory Physiology and Neurobiology, Vol. 167, No. 3, 31.07.2009, p. 323-332.

Research output: Contribution to journalArticle

@article{43b98914922d4ca98f603615dd3c1d8a,
title = "N-Arachidonyl dopamine sensitizes rat capsaicin-sensitive lung vagal afferents via activation of TRPV1 receptors",
abstract = "We investigated the effect of N-arachidonyl dopamine (NADA), an endogenous agonist of both transient receptor potential vanilloid 1 (TRPV1) and cannabinoid CB1 receptors, on the sensitivity of rat capsaicin-sensitive lung vagal afferent (CSLVA) fibers. In artificially ventilated rats, an intravenous infusion of NADA (400 μg/kg/ml, 0.5 ml/min for 2 min) mildly elevated the baseline CSLVA fiber activity, whereas it markedly potentiated CSLVA fiber responses to a right atrial injection of capsaicin or adenosine, and to lung inflation. The potentiating effect on CSLVA fiber sensitivity to an adenosine injection or lung inflation was blocked by capsazepine pretreatment (a TRPV1 receptor antagonist), but was unaffected by AM251 pretreatment (a CB1 receptor antagonist). In spontaneously breathing rats, a NADA infusion similarly potentiated the CSLVA fiber-mediated apneic response evoked by an adenosine injection, and this potentiating effect was also prevented by capsazepine pretreatment. We concluded that NADA at the dose tested non-specifically increases CSLVA fiber sensitivity to chemical and mechanical stimulation via activation of TRPV1 receptors.",
keywords = "Afferent sensitization, Lung, Lung vagal C fibers, NADA, TRPV1 receptors",
author = "Chun-Chun Hsu and Mauo-Ying Bien and Ya-Tien Huang and Ting Ruan and Yu-Ru Kou and You-Shuei Lin",
year = "2009",
month = "7",
day = "31",
doi = "10.1016/j.resp.2009.06.007",
language = "English",
volume = "167",
pages = "323--332",
journal = "Respiratory Physiology and Neurobiology",
issn = "1569-9048",
publisher = "Elsevier Science Publishers B.V.",
number = "3",

}

TY - JOUR

T1 - N-Arachidonyl dopamine sensitizes rat capsaicin-sensitive lung vagal afferents via activation of TRPV1 receptors

AU - Hsu, Chun-Chun

AU - Bien, Mauo-Ying

AU - Huang, Ya-Tien

AU - Ruan, Ting

AU - Kou, Yu-Ru

AU - Lin, You-Shuei

PY - 2009/7/31

Y1 - 2009/7/31

N2 - We investigated the effect of N-arachidonyl dopamine (NADA), an endogenous agonist of both transient receptor potential vanilloid 1 (TRPV1) and cannabinoid CB1 receptors, on the sensitivity of rat capsaicin-sensitive lung vagal afferent (CSLVA) fibers. In artificially ventilated rats, an intravenous infusion of NADA (400 μg/kg/ml, 0.5 ml/min for 2 min) mildly elevated the baseline CSLVA fiber activity, whereas it markedly potentiated CSLVA fiber responses to a right atrial injection of capsaicin or adenosine, and to lung inflation. The potentiating effect on CSLVA fiber sensitivity to an adenosine injection or lung inflation was blocked by capsazepine pretreatment (a TRPV1 receptor antagonist), but was unaffected by AM251 pretreatment (a CB1 receptor antagonist). In spontaneously breathing rats, a NADA infusion similarly potentiated the CSLVA fiber-mediated apneic response evoked by an adenosine injection, and this potentiating effect was also prevented by capsazepine pretreatment. We concluded that NADA at the dose tested non-specifically increases CSLVA fiber sensitivity to chemical and mechanical stimulation via activation of TRPV1 receptors.

AB - We investigated the effect of N-arachidonyl dopamine (NADA), an endogenous agonist of both transient receptor potential vanilloid 1 (TRPV1) and cannabinoid CB1 receptors, on the sensitivity of rat capsaicin-sensitive lung vagal afferent (CSLVA) fibers. In artificially ventilated rats, an intravenous infusion of NADA (400 μg/kg/ml, 0.5 ml/min for 2 min) mildly elevated the baseline CSLVA fiber activity, whereas it markedly potentiated CSLVA fiber responses to a right atrial injection of capsaicin or adenosine, and to lung inflation. The potentiating effect on CSLVA fiber sensitivity to an adenosine injection or lung inflation was blocked by capsazepine pretreatment (a TRPV1 receptor antagonist), but was unaffected by AM251 pretreatment (a CB1 receptor antagonist). In spontaneously breathing rats, a NADA infusion similarly potentiated the CSLVA fiber-mediated apneic response evoked by an adenosine injection, and this potentiating effect was also prevented by capsazepine pretreatment. We concluded that NADA at the dose tested non-specifically increases CSLVA fiber sensitivity to chemical and mechanical stimulation via activation of TRPV1 receptors.

KW - Afferent sensitization

KW - Lung

KW - Lung vagal C fibers

KW - NADA

KW - TRPV1 receptors

UR - http://www.scopus.com/inward/record.url?scp=67651100709&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=67651100709&partnerID=8YFLogxK

U2 - 10.1016/j.resp.2009.06.007

DO - 10.1016/j.resp.2009.06.007

M3 - Article

C2 - 19539789

AN - SCOPUS:67651100709

VL - 167

SP - 323

EP - 332

JO - Respiratory Physiology and Neurobiology

JF - Respiratory Physiology and Neurobiology

SN - 1569-9048

IS - 3

ER -