N-acetylated chitooligosaccharides inhibit TNF-α-induced E-selectin expression in endothelial cells via the JNK-NF-κB pathways

C. W. Lin, L. J. Chen, C. I. Lee, P. L. Lee, J. J. Chiu

Research output: Contribution to journalArticle

Abstract

The anti-inflammatory properties of highly N-acetylated chitooligosaccharides (NACOS) were explored for their potential medical applications. To analyze the effects of NACOS on tumor necrosis factor-α (TNF-α)-induced E-selectin expression, human umbilical vein endothelial cells (ECs) were pre-treated with NACOS with varied concentrations (50, 200, 500 μg/mL) for 1 day before activation by TNF-α for 4 hours. The phosphorylation of different mitogen-activated protein kinases (MAPKs) were examined by Western blot analysis. The effects of NACOS on the E-selectin promoter activity induced by TNF-α were examined by luciferase assay. The chromatin immunoprecipitation (ChIP) assay was performed to investigate the in vivo regulation of nuclear factor-κB (NF-κB) binding to the promoter regions of E-selectin in these ECs by using antibodies against p65 subunit of NF-κB and the promoter-specific primers. Overall, results show that NACOS inhibits TNF-α-induced E-selectin expression in ECs and that this inhibitory effect is mediated through the JNK/NF-κB signaling pathways.

Original languageEnglish
Pages (from-to)165-166
Number of pages2
JournalMCB Molecular and Cellular Biomechanics
Volume3
Issue number4
Publication statusPublished - Dec 1 2006
Externally publishedYes

ASJC Scopus subject areas

  • Biophysics
  • Molecular Medicine
  • Molecular Biology
  • Cell Biology

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