N-α-Acetyltransferase 10 Protein Suppresses Cancer Cell Metastasis by Binding PIX Proteins and Inhibiting Cdc42/Rac1 Activity

Kuo Tai Hua, Ching Ting Tan, Gunnar Johansson, Jang Ming Lee, Pei Wen Yang, Hsin Yi Lu, Chi Kuan Chen, Jen Liang Su, PoShen B. Chen, Yu Ling Wu, Chia Chun Chi, Hsin Jung Kao, Hou Jung Shih, Min Wei Chen, Ming Hsien Chien, Pai Sheng Chen, Wei Jiunn Lee, Tsu Yao Cheng, George Rosenberger, Chee Yin ChaiChih Jen Yang, Ming Shyan Huang, Tsung Ching Lai, Teh Ying Chou, Michael Hsiao, Min Liang Kuo

Research output: Contribution to journalArticle

63 Citations (Scopus)

Abstract

N-α-acetyltransferase 10 protein, Naa10p, is an N-acetyltransferase known to be involved in cell cycle control. We found that Naa10p was expressed lower in varieties of malignancies with lymph node metastasis compared with non-lymph node metastasis. Higher Naa10p expression correlates the survival of lung cancer patients. Naa10p significantly suppressed migration, tumor growth, and metastasis independent of its enzymatic activity. Instead, Naa10p binds to the GIT-binding domain of PIX, thereby preventing the formation of the GIT-PIX-Paxillin complex, resulting in reduced intrinsic Cdc42/Rac1 activity and decreased cell migration. Forced expression of PIX in Naa10-transfected tumor cells restored the migration and metastasis ability. We suggest that Naa10p functions as a tumor metastasis suppressor by disrupting the migratory complex, PIX-GIT- Paxillin, in cancer cells.

Original languageEnglish
Pages (from-to)218-231
Number of pages14
JournalCancer Cell
Volume19
Issue number2
DOIs
Publication statusPublished - Feb 15 2011
Externally publishedYes

ASJC Scopus subject areas

  • Cancer Research
  • Cell Biology
  • Oncology

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  • Cite this

    Hua, K. T., Tan, C. T., Johansson, G., Lee, J. M., Yang, P. W., Lu, H. Y., Chen, C. K., Su, J. L., Chen, P. B., Wu, Y. L., Chi, C. C., Kao, H. J., Shih, H. J., Chen, M. W., Chien, M. H., Chen, P. S., Lee, W. J., Cheng, T. Y., Rosenberger, G., ... Kuo, M. L. (2011). N-α-Acetyltransferase 10 Protein Suppresses Cancer Cell Metastasis by Binding PIX Proteins and Inhibiting Cdc42/Rac1 Activity. Cancer Cell, 19(2), 218-231. https://doi.org/10.1016/j.ccr.2010.11.010