Mycoplasma hyorhinis in Taiwan: Diagnosis and isolation of swine pneumonia pathogen

J. H. Lin, S. P. Chen, K. S. Yeh, C. N. Weng

Research output: Contribution to journalArticle

51 Citations (Scopus)

Abstract

This study attempted to determine whether one multiplex polymerase chain reaction (PCR) is an effective adjunct method for diagnosing Mycoplasma hyopneumoniae and Mycoplasma hyorhinis infection, and whether M. hyorhinis should be considered as an enzootic pneumonia or porcine respiratory disease complex pathogen in Taiwan. To our knowledge, this study is the first to isolate and identify M. hyorhinis as a porcine pathogen in Taiwan. A novel isolation method and a multiplex PCR test were applied to detect and isolate M. hyorhinis. The correlation of M. hyorhinis with swine pneumonia was also examined using a challenge test. Based on weight, 18 pigs were assigned to three groups and housed throughout the study in a specific-pathogen-free (SPF) facility and provided with aseptic feed and water. Groups 1 (n = 6) and 2 (n = 6) were challenged with 5 mL M. hyorhinis culture via tracheal intubation on day 1. The M. hyorhinis strains ATIT-1, -3, and-7 were used to infect group 1 and the strain ATCC 27717 was used for group 2. Culture medium was replaced by phosphate-buffered saline in group 3 (n = 6). All pigs were slaughtered on day 28, and their lungs were removed for examination of lesions. Of the six pigs in group 1 challenged with wild-type strains, two had typical mycoplasma pneumonia lesions. No gross lung lesions were observed in groups 2 and 3. Although further examination is necessary to confirm that wild-type strains can cause pneumonia, it appears that M. hyopneumoniae is no longer the only mycoplasma pathogen implicated in the diagnosis of swine enzootic pneumonia (SEP).

Original languageEnglish
Pages (from-to)111-116
Number of pages6
JournalVeterinary Microbiology
Volume115
Issue number1-3
DOIs
Publication statusPublished - Jun 15 2006

Fingerprint

Mycoplasma hyorhinis
Taiwan
pneumonia
Pneumonia
Swine
swine
pathogens
Mycoplasmal Pneumonia of Swine
Mycoplasma hyopneumoniae
lesions (animal)
Multiplex Polymerase Chain Reaction
Mycoplasma pneumoniae
Swine Diseases
polymerase chain reaction
lungs
Mycoplasma Pneumonia
Mycoplasma Infections
Specific Pathogen-Free Organisms
Lung
Mycoplasma

Keywords

  • M. hyopneumoniae
  • Mycoplasma hyorhinis
  • Swine enzootic pneumonia
  • Taiwan

ASJC Scopus subject areas

  • Animal Science and Zoology
  • Microbiology
  • veterinary(all)

Cite this

Mycoplasma hyorhinis in Taiwan : Diagnosis and isolation of swine pneumonia pathogen. / Lin, J. H.; Chen, S. P.; Yeh, K. S.; Weng, C. N.

In: Veterinary Microbiology, Vol. 115, No. 1-3, 15.06.2006, p. 111-116.

Research output: Contribution to journalArticle

Lin, J. H. ; Chen, S. P. ; Yeh, K. S. ; Weng, C. N. / Mycoplasma hyorhinis in Taiwan : Diagnosis and isolation of swine pneumonia pathogen. In: Veterinary Microbiology. 2006 ; Vol. 115, No. 1-3. pp. 111-116.
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