Mycelial mattress from a sporangia formation-delayed mutant of Rhizopus stoloniferas wound healing-enhancing biomaterial

Mei Yin Chien, Ling Chun Chen, Ying Chen Chen, Ming Thau Sheu, Ya Chi Tsai, Hsiu O. Ho, Ching Hua Su, Der Zen Liu

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

A mycelial mattress of Rhizopus stolonifer obtained from a liquid static culture was utilized for wound dressing and biomedical use. Following screening of mutants induced by UV radiation, F6, exhibiting delayed sporangium formation was selected because its sporangium maturation exhibited a 5-day delay without significant loss of mycelial weight compared to the wild type. The sporangium-free mycelial mattress from the sporangiospore culture of F6 was treated with 1N sodium hydroxide NaOH at 85°C for 2 h to produce a sponge-like membrane named Rhizochitin. The trifluoroacetic acid hydrolysate of Rhizochitin contained 36% N-acetylglucosamine and 53% hexose respectively detected by the Elson-Morgen and phenol-sulfuric acid methods. Results indicated the wound area in rats covered with Rhizochitin was 40% less than that of the uncovered group. Rhizochitin decreased the expression of PDGF in the proliferation stage, increased the expression of TGF-β in the inflammation and proliferation stages, and increased the expression of VEGF in the inflammation and proliferation stages. Rhizochitin inhibited secretion of matrix metalloproteinase-9 on days 1, 7, 9, and 12 and matrix metalloproteinase-2 on days 3, 7, 9, and 12. It was concluded that Rhizochitin has beneficial properties of biocompatible, biodegradable, and wound healing.

Original languageEnglish
Article numbere0134090
JournalPLoS One
Volume10
Issue number8
DOIs
Publication statusPublished - Aug 14 2015

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Sporangia
Rhizopus
Trifluoroacetic Acid
Sodium Hydroxide
biocompatible materials
Hexoses
Acetylglucosamine
Matrix Metalloproteinase 2
sporangia
Matrix Metalloproteinase 9
Biocompatible Materials
Phenol
tissue repair
Ultraviolet radiation
Wound Healing
Vascular Endothelial Growth Factor A
Rats
Screening
plant damage
Membranes

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Mycelial mattress from a sporangia formation-delayed mutant of Rhizopus stoloniferas wound healing-enhancing biomaterial. / Chien, Mei Yin; Chen, Ling Chun; Chen, Ying Chen; Sheu, Ming Thau; Tsai, Ya Chi; Ho, Hsiu O.; Su, Ching Hua; Liu, Der Zen.

In: PLoS One, Vol. 10, No. 8, e0134090, 14.08.2015.

Research output: Contribution to journalArticle

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