Mutational, epigenetic and expressional analyses of caveolin-1 gene in breast cancers.

Shou Tung Chen, Syh Yi Lin, Kun Tu Yeh, Shou Jen Kuo, Wen Ling Chan, Yen Ping Chu, Jan Gowth Chang

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Abstract

Caveolin-1, an integral membrane protein of caveolae found in many cell types, has been suggested as a candidate tumor suppressor. However, the mechanism underlying caveolin-1 decreased expression is not fully understood. The purpose of this study is to investigate the role of aberrant promoter methylation in the regulation of caveolin-1 gene in breast cancer correlated with clinical findings. We used methylation specific PCR, direct sequencing and immunohistochemistry stain methods to explore the role of caveolin-1 gene in the development of breast cancer. We demonstrated that 14 of 55 cases (25.5%) and 4 of 55 cases (7.3%) had methylated CpG-island on caveolin-1 promoter in cancerous and non-cancerous cells, respectively. The frequency of aberrant promoter methylation of breast cancer tissues was significant higher than non-cancerous tissues (p

Original languageEnglish
Pages (from-to)577-582
Number of pages6
JournalInternational Journal of Molecular Medicine
Volume14
Issue number4
Publication statusPublished - Oct 2004
Externally publishedYes

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ASJC Scopus subject areas

  • Genetics

Cite this

Chen, S. T., Lin, S. Y., Yeh, K. T., Kuo, S. J., Chan, W. L., Chu, Y. P., & Chang, J. G. (2004). Mutational, epigenetic and expressional analyses of caveolin-1 gene in breast cancers. International Journal of Molecular Medicine, 14(4), 577-582.