Mutational, epigenetic and expressional analyses of caveolin-1 gene in breast cancers.

Shou Tung Chen; Syh Yi Lin; Kun Tu Yeh; Shou Jen Kuo; Wen Ling Chan; Yen Ping Chu; Jan Gowth Chang

Mutational, epigenetic and expressional analyses of caveolin-1 gene in breast cancers.

Shou Tung Chen, Syh Yi Lin, Kun Tu Yeh, Shou Jen Kuo, Wen Ling Chan, Yen Ping Chu, Jan Gowth Chang

Research output: Contribution to journal › Article › peer-review

Abstract

Caveolin-1, an integral membrane protein of caveolae found in many cell types, has been suggested as a candidate tumor suppressor. However, the mechanism underlying caveolin-1 decreased expression is not fully understood. The purpose of this study is to investigate the role of aberrant promoter methylation in the regulation of caveolin-1 gene in breast cancer correlated with clinical findings. We used methylation specific PCR, direct sequencing and immunohistochemistry stain methods to explore the role of caveolin-1 gene in the development of breast cancer. We demonstrated that 14 of 55 cases (25.5%) and 4 of 55 cases (7.3%) had methylated CpG-island on caveolin-1 promoter in cancerous and non-cancerous cells, respectively. The frequency of aberrant promoter methylation of breast cancer tissues was significant higher than non-cancerous tissues (p

Original language	English
Pages (from-to)	577-582
Number of pages	6
Journal	International Journal of Molecular Medicine
Volume	14
Issue number	4
Publication status	Published - Oct 2004
Externally published	Yes

ASJC Scopus subject areas

Genetics

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title = "Mutational, epigenetic and expressional analyses of caveolin-1 gene in breast cancers.",

abstract = "Caveolin-1, an integral membrane protein of caveolae found in many cell types, has been suggested as a candidate tumor suppressor. However, the mechanism underlying caveolin-1 decreased expression is not fully understood. The purpose of this study is to investigate the role of aberrant promoter methylation in the regulation of caveolin-1 gene in breast cancer correlated with clinical findings. We used methylation specific PCR, direct sequencing and immunohistochemistry stain methods to explore the role of caveolin-1 gene in the development of breast cancer. We demonstrated that 14 of 55 cases (25.5%) and 4 of 55 cases (7.3%) had methylated CpG-island on caveolin-1 promoter in cancerous and non-cancerous cells, respectively. The frequency of aberrant promoter methylation of breast cancer tissues was significant higher than non-cancerous tissues (p",

author = "Chen, {Shou Tung} and Lin, {Syh Yi} and Yeh, {Kun Tu} and Kuo, {Shou Jen} and Chan, {Wen Ling} and Chu, {Yen Ping} and Chang, {Jan Gowth}",

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T1 - Mutational, epigenetic and expressional analyses of caveolin-1 gene in breast cancers.

AU - Chen, Shou Tung

AU - Lin, Syh Yi

AU - Yeh, Kun Tu

AU - Kuo, Shou Jen

AU - Chan, Wen Ling

AU - Chu, Yen Ping

AU - Chang, Jan Gowth

PY - 2004/10

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AB - Caveolin-1, an integral membrane protein of caveolae found in many cell types, has been suggested as a candidate tumor suppressor. However, the mechanism underlying caveolin-1 decreased expression is not fully understood. The purpose of this study is to investigate the role of aberrant promoter methylation in the regulation of caveolin-1 gene in breast cancer correlated with clinical findings. We used methylation specific PCR, direct sequencing and immunohistochemistry stain methods to explore the role of caveolin-1 gene in the development of breast cancer. We demonstrated that 14 of 55 cases (25.5%) and 4 of 55 cases (7.3%) had methylated CpG-island on caveolin-1 promoter in cancerous and non-cancerous cells, respectively. The frequency of aberrant promoter methylation of breast cancer tissues was significant higher than non-cancerous tissues (p

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