Mutational, epigenetic and expressional analyses of caveolin-1 gene in breast cancers.

Shou Tung Chen, Syh Yi Lin, Kun Tu Yeh, Shou Jen Kuo, Wen Ling Chan, Yen Ping Chu, Jan Gowth Chang

Research output: Contribution to journalArticle

55 Citations (Scopus)

Abstract

Caveolin-1, an integral membrane protein of caveolae found in many cell types, has been suggested as a candidate tumor suppressor. However, the mechanism underlying caveolin-1 decreased expression is not fully understood. The purpose of this study is to investigate the role of aberrant promoter methylation in the regulation of caveolin-1 gene in breast cancer correlated with clinical findings. We used methylation specific PCR, direct sequencing and immunohistochemistry stain methods to explore the role of caveolin-1 gene in the development of breast cancer. We demonstrated that 14 of 55 cases (25.5%) and 4 of 55 cases (7.3%) had methylated CpG-island on caveolin-1 promoter in cancerous and non-cancerous cells, respectively. The frequency of aberrant promoter methylation of breast cancer tissues was significant higher than non-cancerous tissues (p

Original languageEnglish
Pages (from-to)577-582
Number of pages6
JournalInternational Journal of Molecular Medicine
Volume14
Issue number4
Publication statusPublished - Oct 2004
Externally publishedYes

Fingerprint

Caveolin 1
Epigenomics
Breast Neoplasms
Methylation
Genes
Caveolae
CpG Islands
Membrane Proteins
Coloring Agents
Immunohistochemistry
Polymerase Chain Reaction
Neoplasms

ASJC Scopus subject areas

  • Genetics

Cite this

Chen, S. T., Lin, S. Y., Yeh, K. T., Kuo, S. J., Chan, W. L., Chu, Y. P., & Chang, J. G. (2004). Mutational, epigenetic and expressional analyses of caveolin-1 gene in breast cancers. International Journal of Molecular Medicine, 14(4), 577-582.

Mutational, epigenetic and expressional analyses of caveolin-1 gene in breast cancers. / Chen, Shou Tung; Lin, Syh Yi; Yeh, Kun Tu; Kuo, Shou Jen; Chan, Wen Ling; Chu, Yen Ping; Chang, Jan Gowth.

In: International Journal of Molecular Medicine, Vol. 14, No. 4, 10.2004, p. 577-582.

Research output: Contribution to journalArticle

Chen, ST, Lin, SY, Yeh, KT, Kuo, SJ, Chan, WL, Chu, YP & Chang, JG 2004, 'Mutational, epigenetic and expressional analyses of caveolin-1 gene in breast cancers.', International Journal of Molecular Medicine, vol. 14, no. 4, pp. 577-582.
Chen, Shou Tung ; Lin, Syh Yi ; Yeh, Kun Tu ; Kuo, Shou Jen ; Chan, Wen Ling ; Chu, Yen Ping ; Chang, Jan Gowth. / Mutational, epigenetic and expressional analyses of caveolin-1 gene in breast cancers. In: International Journal of Molecular Medicine. 2004 ; Vol. 14, No. 4. pp. 577-582.
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