Abstract
Caveolin-1, an integral membrane protein of caveolae found in many cell types, has been suggested as a candidate tumor suppressor. However, the mechanism underlying caveolin-1 decreased expression is not fully understood. The purpose of this study is to investigate the role of aberrant promoter methylation in the regulation of caveolin-1 gene in breast cancer correlated with clinical findings. We used methylation specific PCR, direct sequencing and immunohistochemistry stain methods to explore the role of caveolin-1 gene in the development of breast cancer. We demonstrated that 14 of 55 cases (25.5%) and 4 of 55 cases (7.3%) had methylated CpG-island on caveolin-1 promoter in cancerous and non-cancerous cells, respectively. The frequency of aberrant promoter methylation of breast cancer tissues was significant higher than non-cancerous tissues (p
| Original language | English |
|---|---|
| Pages (from-to) | 577-582 |
| Number of pages | 6 |
| Journal | International Journal of Molecular Medicine |
| Volume | 14 |
| Issue number | 4 |
| Publication status | Published - Oct 2004 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Genetics
Cite this
Mutational, epigenetic and expressional analyses of caveolin-1 gene in breast cancers. / Chen, Shou Tung; Lin, Syh Yi; Yeh, Kun Tu; Kuo, Shou Jen; Chan, Wen Ling; Chu, Yen Ping; Chang, Jan Gowth.
In: International Journal of Molecular Medicine, Vol. 14, No. 4, 10.2004, p. 577-582.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Mutational, epigenetic and expressional analyses of caveolin-1 gene in breast cancers.
AU - Chen, Shou Tung
AU - Lin, Syh Yi
AU - Yeh, Kun Tu
AU - Kuo, Shou Jen
AU - Chan, Wen Ling
AU - Chu, Yen Ping
AU - Chang, Jan Gowth
PY - 2004/10
Y1 - 2004/10
N2 - Caveolin-1, an integral membrane protein of caveolae found in many cell types, has been suggested as a candidate tumor suppressor. However, the mechanism underlying caveolin-1 decreased expression is not fully understood. The purpose of this study is to investigate the role of aberrant promoter methylation in the regulation of caveolin-1 gene in breast cancer correlated with clinical findings. We used methylation specific PCR, direct sequencing and immunohistochemistry stain methods to explore the role of caveolin-1 gene in the development of breast cancer. We demonstrated that 14 of 55 cases (25.5%) and 4 of 55 cases (7.3%) had methylated CpG-island on caveolin-1 promoter in cancerous and non-cancerous cells, respectively. The frequency of aberrant promoter methylation of breast cancer tissues was significant higher than non-cancerous tissues (p
AB - Caveolin-1, an integral membrane protein of caveolae found in many cell types, has been suggested as a candidate tumor suppressor. However, the mechanism underlying caveolin-1 decreased expression is not fully understood. The purpose of this study is to investigate the role of aberrant promoter methylation in the regulation of caveolin-1 gene in breast cancer correlated with clinical findings. We used methylation specific PCR, direct sequencing and immunohistochemistry stain methods to explore the role of caveolin-1 gene in the development of breast cancer. We demonstrated that 14 of 55 cases (25.5%) and 4 of 55 cases (7.3%) had methylated CpG-island on caveolin-1 promoter in cancerous and non-cancerous cells, respectively. The frequency of aberrant promoter methylation of breast cancer tissues was significant higher than non-cancerous tissues (p
UR - http://www.scopus.com/inward/record.url?scp=16644374354&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=16644374354&partnerID=8YFLogxK
M3 - Article
C2 - 15375584
AN - SCOPUS:16644374354
VL - 14
SP - 577
EP - 582
JO - International Journal of Molecular Medicine
JF - International Journal of Molecular Medicine
SN - 1107-3756
IS - 4
ER -