Mutational analysis of the p27(kip1) gene in hepatocellular carcinoma

Tse Ching Chen, Kwai Fong Ng, Jau Min Lien, Long Bin Jeng, Miin Fu Chen, Ling Ling Hsieh

Research output: Contribution to journalArticlepeer-review

18 Citations (Scopus)

Abstract

p27(Kip1) is an inhibitor of cyclin-dependent kinase. It has been reported that reduced p27(Kip1) expression is present in human hepatocellular carcinoma. To determine the role of p27(Kip1) in hepatocarcinogenesis, 46 cases with hepatocellular carcinomas were studied. p27(Kip1) mutation was first screened by single strand conformation polymorphism, and direct DNA sequencing was then performed on those cases with mobility shifts. Two polymorphism sites were found. One is a previously described polymorphism at codon 109 (GTC→GGC) which was found in two cases. The second polymorphism was identified at codon 55 (GCG→GCA) in six of the 46 cases. However, the polymorphism at codon 55 was also present in seven of 93 healthy controls (7.5%), indicating that it is not associated with a predisposition for development of hepatocellular carcinoma (Fisher's exact test, P>0.05). These results show that p27(Kip1) mutation is not a frequent event in human hepatocellular carcinoma, and suggest that it may be inactivated predominantly by transcriptional and/or posttranscriptional regulation rather than genomic aberrations. Copyright (C) 2000 Elsevier Science Ireland Ltd.

Original languageEnglish
Pages (from-to)169-173
Number of pages5
JournalCancer Letters
Volume153
Issue number1-2
DOIs
Publication statusPublished - May 29 2000
Externally publishedYes

Keywords

  • Hepatocellular carcinoma
  • Kip1
  • p27
  • Single strand conformation polymorphism

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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