Mutation analysis of heat shock cognate protein 70 gene in colorectal cancer

Ruey H. Kao, Ming Hsun Kuo

Research output: Contribution to journalArticle

Abstract

Objective: The aim of the study is to investigate possible mutations in heat shock cognate 70 gene (HSC70) in colorectal cancer. Materials and Methods: DNA was extracted from frozen tissue sections of 10 colorectal cancers and peripheral blood cells of 10 healthy people. The exons of HSC70 were PCR-amplified with 4 pairs of primers. The PCR products were run along a 1.5% agarose gel containing ethidium bromide and visualised under UV illumination. The PCR products were purified from the gel and direct-sequenced. The sequences were analysed according to GenBank access number Y00371. Results: Ten genetic alterations were found, and 6 were novel findings. Nine of them occurred both in colorectal cancer and healthy subjects, indicating polymorphisms. The remaining one, D6, found only in two colorectal cancers (20%), occurred at sequence 3252 in exon 6, but this sequence change did not alter the reading frame of HSC70 because of the third position wobble in codon 423. Conclusions: In the present study no significant genetic alterations were found in HSC70 for colorectal cancer; however several novel polymorphisms found only in this study demonstrated genomic differences between Asian and Western populations.

Original languageEnglish
Pages (from-to)347-351
Number of pages5
JournalTzu Chi Medical Journal
Volume14
Issue number6
Publication statusPublished - Dec 1 2002
Externally publishedYes

Fingerprint

HSC70 Heat-Shock Proteins
Colorectal Neoplasms
Shock
Mutation
Hot Temperature
Genes
Polymerase Chain Reaction
Exons
Gels
Reading Frames
Ethidium
Nucleic Acid Databases
Frozen Sections
Lighting
Codon
Sepharose
Blood Cells
Healthy Volunteers
DNA
Population

Keywords

  • Colorectal cancer
  • Heat shock cognate protein 70
  • Polymorphism

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Mutation analysis of heat shock cognate protein 70 gene in colorectal cancer. / Kao, Ruey H.; Kuo, Ming Hsun.

In: Tzu Chi Medical Journal, Vol. 14, No. 6, 01.12.2002, p. 347-351.

Research output: Contribution to journalArticle

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