Muscle type from which satellite cells are derived plays a role in their damage response

Chuang Yu Lin, Chun Yin Hou, Chung Min Tsai, Hsi Chang, Hsi Chang

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)

Abstract

The aim of this study was to evaluate the response of satellite cells to muscular atrophies which possess different pathological characteristics and which were induced by distinct damages. Right lower limbs of rats were exposed to denervation or disuse and later its tibialis anterior (TA) or soleus (SOL) muscles were analyzed. After confirming their functional impairments indicated by common but distinct pathological and electrophysiological characteristics, the quantitative polymerase chain reaction analysis of Pax7 and Pax3 expressions and the number of Pax7+veand Pax3+vecells were analyzed sequentially at day 0, day 7, and day 14. TA muscles of both denervation-and disuse-induced atrophy models showed persisted low level of Pax7 expression from day 7 (0.91 ± 0.23 and 0.31 ± 0.07, P = 0.06, n = 6) through day 14 (1.09 ± 0.15 and 0.4 ± 0.09 [P < 0.05]). On the other hand, significant elevations were observed in Pax3 expression in both atrophy models (2.73 ± 0.46 and 2.75 ± 0.26 [P < 0.05]) at day 7. Similar to TA muscle, resembled pattern of Pax7 and Pax3 expression changes were observed between the SOL muscles of denervation-and disused-atrophy models. These trends were further confirmed by the changes in Pax7+veand Pax3+vecell numbers of TA and SOL muscles in both atrophy models. Despite the distinct pathological findings, similar patterns in the changes of Pax3 and Pax7 expressions and the changes of Pax7+veand Pax3+vecell numbers were observed between the denervation-and disuse-induced atrophy models and this commonality was admitted among the muscle type. Therefore, we claim that the muscle regeneration orchestrated by satellite cells was governed by the muscle type in which satellite cells reside.

Original languageEnglish
Pages (from-to)113-121
Number of pages9
JournalChinese Journal of Physiology
Volume63
Issue number3
DOIs
Publication statusPublished - May 1 2020

Keywords

  • Denervation-induced atrophy model
  • disuse-induced atrophy model
  • muscle regeneration
  • muscle type
  • Pax3 and Pax7

ASJC Scopus subject areas

  • Physiology
  • Physiology (medical)

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