Multiplex detection of urinary miRNA biomarkers by transmission surface plasmon resonance

Wing Kiu Yeung, Huai Yi Chen, Juan Jie Sun, Tung Han Hsieh, Mansoureh Z. Mousavi, Hsi Hsien Chen, Kuang Li Lee, Heng Lin, Pei Kuen Wei, Ji Yen Cheng

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

The clinical assessment of short-stranded nucleic acid biomarkers such as miRNAs could potentially provide useful information for monitoring disease progression, prompting definitive treatment decisions. In the past decade, advancements in biosensing technology have led to a shift towards rapid, real-time and label-free detection systems; as such, surface plasmon resonance (SPR) biosensor-based technology has become of high interest. Here, we developed an automated multiplex transmissive surface plasmon resonance (t-SPR) platform with the use of a capped gold nanoslit integrated microfluidic surface plasmon resonance (SPR) biosensor. The automated platform was custom designed to allow the analysis of spectral measurements using wavelength shift (dλ), intensity (dI) and novel area change (dA) for surface binding reactions. A simple and compact nanostructure based biosensor was fabricated with multiplex real-time detection capabilities. The sensitivity and specificity of the microfluidic device was demonstrated through the use of functionalised AuNPs for target molecule isolation and signal enhancement in combination with probes on the CG nanoslit surface. Our work allows for the multiplex detection of miRNA at femtomolar concentrations in complex media such as urine.

Original languageEnglish
Pages (from-to)4715-4722
Number of pages8
JournalAnalyst
Volume143
Issue number19
DOIs
Publication statusPublished - Oct 7 2018

Fingerprint

Surface Plasmon Resonance
Surface plasmon resonance
Biosensing Techniques
Biomarkers
MicroRNAs
Biosensors
biomarker
Lab-On-A-Chip Devices
Microfluidics
Technology
Nanostructures
Nucleic acids
Gold
Nucleic Acids
Disease Progression
Labels
Urine
Sensitivity and Specificity
nucleic acid
Wavelength

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Environmental Chemistry
  • Spectroscopy
  • Electrochemistry

Cite this

Yeung, W. K., Chen, H. Y., Sun, J. J., Hsieh, T. H., Mousavi, M. Z., Chen, H. H., ... Cheng, J. Y. (2018). Multiplex detection of urinary miRNA biomarkers by transmission surface plasmon resonance. Analyst, 143(19), 4715-4722. https://doi.org/10.1039/c8an01127c

Multiplex detection of urinary miRNA biomarkers by transmission surface plasmon resonance. / Yeung, Wing Kiu; Chen, Huai Yi; Sun, Juan Jie; Hsieh, Tung Han; Mousavi, Mansoureh Z.; Chen, Hsi Hsien; Lee, Kuang Li; Lin, Heng; Wei, Pei Kuen; Cheng, Ji Yen.

In: Analyst, Vol. 143, No. 19, 07.10.2018, p. 4715-4722.

Research output: Contribution to journalArticle

Yeung, WK, Chen, HY, Sun, JJ, Hsieh, TH, Mousavi, MZ, Chen, HH, Lee, KL, Lin, H, Wei, PK & Cheng, JY 2018, 'Multiplex detection of urinary miRNA biomarkers by transmission surface plasmon resonance', Analyst, vol. 143, no. 19, pp. 4715-4722. https://doi.org/10.1039/c8an01127c
Yeung, Wing Kiu ; Chen, Huai Yi ; Sun, Juan Jie ; Hsieh, Tung Han ; Mousavi, Mansoureh Z. ; Chen, Hsi Hsien ; Lee, Kuang Li ; Lin, Heng ; Wei, Pei Kuen ; Cheng, Ji Yen. / Multiplex detection of urinary miRNA biomarkers by transmission surface plasmon resonance. In: Analyst. 2018 ; Vol. 143, No. 19. pp. 4715-4722.
@article{bfeda1112001473e9c6d59cad642910f,
title = "Multiplex detection of urinary miRNA biomarkers by transmission surface plasmon resonance",
abstract = "The clinical assessment of short-stranded nucleic acid biomarkers such as miRNAs could potentially provide useful information for monitoring disease progression, prompting definitive treatment decisions. In the past decade, advancements in biosensing technology have led to a shift towards rapid, real-time and label-free detection systems; as such, surface plasmon resonance (SPR) biosensor-based technology has become of high interest. Here, we developed an automated multiplex transmissive surface plasmon resonance (t-SPR) platform with the use of a capped gold nanoslit integrated microfluidic surface plasmon resonance (SPR) biosensor. The automated platform was custom designed to allow the analysis of spectral measurements using wavelength shift (dλ), intensity (dI) and novel area change (dA) for surface binding reactions. A simple and compact nanostructure based biosensor was fabricated with multiplex real-time detection capabilities. The sensitivity and specificity of the microfluidic device was demonstrated through the use of functionalised AuNPs for target molecule isolation and signal enhancement in combination with probes on the CG nanoslit surface. Our work allows for the multiplex detection of miRNA at femtomolar concentrations in complex media such as urine.",
author = "Yeung, {Wing Kiu} and Chen, {Huai Yi} and Sun, {Juan Jie} and Hsieh, {Tung Han} and Mousavi, {Mansoureh Z.} and Chen, {Hsi Hsien} and Lee, {Kuang Li} and Heng Lin and Wei, {Pei Kuen} and Cheng, {Ji Yen}",
year = "2018",
month = "10",
day = "7",
doi = "10.1039/c8an01127c",
language = "English",
volume = "143",
pages = "4715--4722",
journal = "The Analyst",
issn = "0003-2654",
publisher = "Royal Society of Chemistry",
number = "19",

}

TY - JOUR

T1 - Multiplex detection of urinary miRNA biomarkers by transmission surface plasmon resonance

AU - Yeung, Wing Kiu

AU - Chen, Huai Yi

AU - Sun, Juan Jie

AU - Hsieh, Tung Han

AU - Mousavi, Mansoureh Z.

AU - Chen, Hsi Hsien

AU - Lee, Kuang Li

AU - Lin, Heng

AU - Wei, Pei Kuen

AU - Cheng, Ji Yen

PY - 2018/10/7

Y1 - 2018/10/7

N2 - The clinical assessment of short-stranded nucleic acid biomarkers such as miRNAs could potentially provide useful information for monitoring disease progression, prompting definitive treatment decisions. In the past decade, advancements in biosensing technology have led to a shift towards rapid, real-time and label-free detection systems; as such, surface plasmon resonance (SPR) biosensor-based technology has become of high interest. Here, we developed an automated multiplex transmissive surface plasmon resonance (t-SPR) platform with the use of a capped gold nanoslit integrated microfluidic surface plasmon resonance (SPR) biosensor. The automated platform was custom designed to allow the analysis of spectral measurements using wavelength shift (dλ), intensity (dI) and novel area change (dA) for surface binding reactions. A simple and compact nanostructure based biosensor was fabricated with multiplex real-time detection capabilities. The sensitivity and specificity of the microfluidic device was demonstrated through the use of functionalised AuNPs for target molecule isolation and signal enhancement in combination with probes on the CG nanoslit surface. Our work allows for the multiplex detection of miRNA at femtomolar concentrations in complex media such as urine.

AB - The clinical assessment of short-stranded nucleic acid biomarkers such as miRNAs could potentially provide useful information for monitoring disease progression, prompting definitive treatment decisions. In the past decade, advancements in biosensing technology have led to a shift towards rapid, real-time and label-free detection systems; as such, surface plasmon resonance (SPR) biosensor-based technology has become of high interest. Here, we developed an automated multiplex transmissive surface plasmon resonance (t-SPR) platform with the use of a capped gold nanoslit integrated microfluidic surface plasmon resonance (SPR) biosensor. The automated platform was custom designed to allow the analysis of spectral measurements using wavelength shift (dλ), intensity (dI) and novel area change (dA) for surface binding reactions. A simple and compact nanostructure based biosensor was fabricated with multiplex real-time detection capabilities. The sensitivity and specificity of the microfluidic device was demonstrated through the use of functionalised AuNPs for target molecule isolation and signal enhancement in combination with probes on the CG nanoslit surface. Our work allows for the multiplex detection of miRNA at femtomolar concentrations in complex media such as urine.

UR - http://www.scopus.com/inward/record.url?scp=85053931587&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85053931587&partnerID=8YFLogxK

U2 - 10.1039/c8an01127c

DO - 10.1039/c8an01127c

M3 - Article

C2 - 30188550

AN - SCOPUS:85053931587

VL - 143

SP - 4715

EP - 4722

JO - The Analyst

JF - The Analyst

SN - 0003-2654

IS - 19

ER -