Multiplex detection of urinary miRNA biomarkers by transmission surface plasmon resonance

Wing Kiu Yeung, Huai Yi Chen, Juan Jie Sun, Tung Han Hsieh, Mansoureh Z. Mousavi, Hsi Hsien Chen, Kuang Li Lee, Heng Lin, Pei Kuen Wei, Ji Yen Cheng

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

The clinical assessment of short-stranded nucleic acid biomarkers such as miRNAs could potentially provide useful information for monitoring disease progression, prompting definitive treatment decisions. In the past decade, advancements in biosensing technology have led to a shift towards rapid, real-time and label-free detection systems; as such, surface plasmon resonance (SPR) biosensor-based technology has become of high interest. Here, we developed an automated multiplex transmissive surface plasmon resonance (t-SPR) platform with the use of a capped gold nanoslit integrated microfluidic surface plasmon resonance (SPR) biosensor. The automated platform was custom designed to allow the analysis of spectral measurements using wavelength shift (dλ), intensity (dI) and novel area change (dA) for surface binding reactions. A simple and compact nanostructure based biosensor was fabricated with multiplex real-time detection capabilities. The sensitivity and specificity of the microfluidic device was demonstrated through the use of functionalised AuNPs for target molecule isolation and signal enhancement in combination with probes on the CG nanoslit surface. Our work allows for the multiplex detection of miRNA at femtomolar concentrations in complex media such as urine.

Original languageEnglish
Pages (from-to)4715-4722
Number of pages8
JournalAnalyst
Volume143
Issue number19
DOIs
Publication statusPublished - Oct 7 2018

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Environmental Chemistry
  • Spectroscopy
  • Electrochemistry

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  • Cite this

    Yeung, W. K., Chen, H. Y., Sun, J. J., Hsieh, T. H., Mousavi, M. Z., Chen, H. H., Lee, K. L., Lin, H., Wei, P. K., & Cheng, J. Y. (2018). Multiplex detection of urinary miRNA biomarkers by transmission surface plasmon resonance. Analyst, 143(19), 4715-4722. https://doi.org/10.1039/c8an01127c