Abstract
Objective: To determine the rearrangement of mitochondrial DNA (mtDNA) in unfertilized human oocytes and compromised embryos to evaluate the fertilization capacity of oocytes. Design: Prospective laboratory research. Setting: IVF laboratory in a university hospital. Patient(s): One hundred twenty-four unfertilized oocytes, 98 arrested embryos, and 45 tripronucleate (3PN) embryos from 65 female patients undergoing in vitro fertilization (IVF). Intervention(s): Unfertilized oocytes and poor quality embryos were collected 48 hours after IVF. Main Outcome Measure(s): Comparison of the frequency of mtDNA deletions and fertilization rates of oocytes. Result(s): Multiple deletions of mtDNA were found in unfertilized oocytes and arrested embryos obtained from IVF patients. A 4977-bp deletion was the most frequent deletion in human oocytes and embryos. About 66.1% of the unfertilized oocytes, 34.8% of the arrested or fragmented embryos, and 21.1% of the 3PN embryos harbored the 4977-bp deletion of mtDNA. There was a significant increase in the proportion of deleted mtDNA in unfertilized oocytes. Conclusion(s): Accumulation of mtDNA deletions may contribute to mitochondrial dysfunction and impaired ATP production. We conclude that the accumulation of rearranged mtDNA may interfere with fertilization of human oocytes and further embryonic development.
| Original language | English |
|---|---|
| Pages (from-to) | 1012-1017 |
| Number of pages | 6 |
| Journal | Fertility and Sterility |
| Volume | 77 |
| Issue number | 5 |
| DOIs | |
| Publication status | Published - 2002 |
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Keywords
- Embryo
- Infertility
- mtDNA rearrangement
- Oocyte
- Tripronucleate
ASJC Scopus subject areas
- Obstetrics and Gynaecology
Cite this
Multiple rearrangements of mitochondrial DNA in unfertilized human oocytes. / Hsieh, Rong Hong; Tsai, Nu Man; Au, Heng Kien; Chang, Shu Ju; Wei, Yau Huei; Tzeng, Chii Ruey.
In: Fertility and Sterility, Vol. 77, No. 5, 2002, p. 1012-1017.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Multiple rearrangements of mitochondrial DNA in unfertilized human oocytes
AU - Hsieh, Rong Hong
AU - Tsai, Nu Man
AU - Au, Heng Kien
AU - Chang, Shu Ju
AU - Wei, Yau Huei
AU - Tzeng, Chii Ruey
PY - 2002
Y1 - 2002
N2 - Objective: To determine the rearrangement of mitochondrial DNA (mtDNA) in unfertilized human oocytes and compromised embryos to evaluate the fertilization capacity of oocytes. Design: Prospective laboratory research. Setting: IVF laboratory in a university hospital. Patient(s): One hundred twenty-four unfertilized oocytes, 98 arrested embryos, and 45 tripronucleate (3PN) embryos from 65 female patients undergoing in vitro fertilization (IVF). Intervention(s): Unfertilized oocytes and poor quality embryos were collected 48 hours after IVF. Main Outcome Measure(s): Comparison of the frequency of mtDNA deletions and fertilization rates of oocytes. Result(s): Multiple deletions of mtDNA were found in unfertilized oocytes and arrested embryos obtained from IVF patients. A 4977-bp deletion was the most frequent deletion in human oocytes and embryos. About 66.1% of the unfertilized oocytes, 34.8% of the arrested or fragmented embryos, and 21.1% of the 3PN embryos harbored the 4977-bp deletion of mtDNA. There was a significant increase in the proportion of deleted mtDNA in unfertilized oocytes. Conclusion(s): Accumulation of mtDNA deletions may contribute to mitochondrial dysfunction and impaired ATP production. We conclude that the accumulation of rearranged mtDNA may interfere with fertilization of human oocytes and further embryonic development.
AB - Objective: To determine the rearrangement of mitochondrial DNA (mtDNA) in unfertilized human oocytes and compromised embryos to evaluate the fertilization capacity of oocytes. Design: Prospective laboratory research. Setting: IVF laboratory in a university hospital. Patient(s): One hundred twenty-four unfertilized oocytes, 98 arrested embryos, and 45 tripronucleate (3PN) embryos from 65 female patients undergoing in vitro fertilization (IVF). Intervention(s): Unfertilized oocytes and poor quality embryos were collected 48 hours after IVF. Main Outcome Measure(s): Comparison of the frequency of mtDNA deletions and fertilization rates of oocytes. Result(s): Multiple deletions of mtDNA were found in unfertilized oocytes and arrested embryos obtained from IVF patients. A 4977-bp deletion was the most frequent deletion in human oocytes and embryos. About 66.1% of the unfertilized oocytes, 34.8% of the arrested or fragmented embryos, and 21.1% of the 3PN embryos harbored the 4977-bp deletion of mtDNA. There was a significant increase in the proportion of deleted mtDNA in unfertilized oocytes. Conclusion(s): Accumulation of mtDNA deletions may contribute to mitochondrial dysfunction and impaired ATP production. We conclude that the accumulation of rearranged mtDNA may interfere with fertilization of human oocytes and further embryonic development.
KW - Embryo
KW - Infertility
KW - mtDNA rearrangement
KW - Oocyte
KW - Tripronucleate
UR - http://www.scopus.com/inward/record.url?scp=0036260765&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0036260765&partnerID=8YFLogxK
U2 - 10.1016/S0015-0282(02)02994-1
DO - 10.1016/S0015-0282(02)02994-1
M3 - Article
C2 - 12009360
AN - SCOPUS:0036260765
VL - 77
SP - 1012
EP - 1017
JO - Fertility and Sterility
JF - Fertility and Sterility
SN - 0015-0282
IS - 5
ER -