Multiple rearrangements of mitochondrial DNA in unfertilized human oocytes

Rong Hong Hsieh, Nu Man Tsai, Heng Kien Au, Shu Ju Chang, Yau Huei Wei, Chii Ruey Tzeng

Research output: Contribution to journalArticle

48 Citations (Scopus)

Abstract

Objective: To determine the rearrangement of mitochondrial DNA (mtDNA) in unfertilized human oocytes and compromised embryos to evaluate the fertilization capacity of oocytes. Design: Prospective laboratory research. Setting: IVF laboratory in a university hospital. Patient(s): One hundred twenty-four unfertilized oocytes, 98 arrested embryos, and 45 tripronucleate (3PN) embryos from 65 female patients undergoing in vitro fertilization (IVF). Intervention(s): Unfertilized oocytes and poor quality embryos were collected 48 hours after IVF. Main Outcome Measure(s): Comparison of the frequency of mtDNA deletions and fertilization rates of oocytes. Result(s): Multiple deletions of mtDNA were found in unfertilized oocytes and arrested embryos obtained from IVF patients. A 4977-bp deletion was the most frequent deletion in human oocytes and embryos. About 66.1% of the unfertilized oocytes, 34.8% of the arrested or fragmented embryos, and 21.1% of the 3PN embryos harbored the 4977-bp deletion of mtDNA. There was a significant increase in the proportion of deleted mtDNA in unfertilized oocytes. Conclusion(s): Accumulation of mtDNA deletions may contribute to mitochondrial dysfunction and impaired ATP production. We conclude that the accumulation of rearranged mtDNA may interfere with fertilization of human oocytes and further embryonic development.

Original languageEnglish
Pages (from-to)1012-1017
Number of pages6
JournalFertility and Sterility
Volume77
Issue number5
DOIs
Publication statusPublished - 2002

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Keywords

  • Embryo
  • Infertility
  • mtDNA rearrangement
  • Oocyte
  • Tripronucleate

ASJC Scopus subject areas

  • Obstetrics and Gynaecology

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