TY - JOUR
T1 - Multiple myeloma driving factor WHSC1 is a transcription target of oncogene HMGA2 that facilitates colon cancer proliferation and metastasis
AU - Liu, Hou Hsien
AU - Lee, Chia Hwa
AU - Hsieh, Yi Chen
AU - Hsu, Duen Wei
AU - Cho, Er Chieh
N1 - Funding Information:
This project was supported by the Ministry of Science and Technology ( MOST107-2320-B-038-054 , MOST109-2320-B-038-038 , and MOST109-2314-B-152-001 ) and WanFang Hospital, Chi-Mei Medical Center , and Hualien Tzu-Chi Hospital Joint Cancer Center Grant-Focus on Colon Cancer Research ( MOHW109-TDU-B-212-134020 , supported by Health and welfare surcharge of tobacco products), Taiwan.
Publisher Copyright:
© 2021 Elsevier Inc.
PY - 2021/8/27
Y1 - 2021/8/27
N2 - Colon cancer is a common human cancer worldwide. The survival rate of late staged or metastatic colon cancer patients remains low even though the effectiveness of treatment in colon cancer has greatly improved. Research on tumorigenesis mechanisms and discovery of novel molecular target for treating colon cancer is critical. The promotion roles of WHSC1 in multiple myeloma have been demonstrated previously, yet, the regulation of WHSC1 in other cancers is largely unknown, especially in colon cancer. Here, in this study, we analyzed and identified WHSC1 while studying the genetic regulations of HMGA2 in colon cancer cells by microarray analysis, and investigated the HMGA2-WHSC1 interaction. We then applied CRISPR technology to establish stable WHSC1 knockout cells, to address the functional regulation of WHSC1 in colon cancer. In summary, our results for the first time identified the HMGA2-WHSC1 interaction in colon cancer. Moreover, we discovered that WHSC1 promotes cancer proliferation, facilitates resistance of chemotherapy agent, and promotes metastatic capacity of colon cancer.
AB - Colon cancer is a common human cancer worldwide. The survival rate of late staged or metastatic colon cancer patients remains low even though the effectiveness of treatment in colon cancer has greatly improved. Research on tumorigenesis mechanisms and discovery of novel molecular target for treating colon cancer is critical. The promotion roles of WHSC1 in multiple myeloma have been demonstrated previously, yet, the regulation of WHSC1 in other cancers is largely unknown, especially in colon cancer. Here, in this study, we analyzed and identified WHSC1 while studying the genetic regulations of HMGA2 in colon cancer cells by microarray analysis, and investigated the HMGA2-WHSC1 interaction. We then applied CRISPR technology to establish stable WHSC1 knockout cells, to address the functional regulation of WHSC1 in colon cancer. In summary, our results for the first time identified the HMGA2-WHSC1 interaction in colon cancer. Moreover, we discovered that WHSC1 promotes cancer proliferation, facilitates resistance of chemotherapy agent, and promotes metastatic capacity of colon cancer.
KW - Colon cancer
KW - HMGA2
KW - Metastasis
KW - Transcription regulation
KW - WHSC1
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UR - http://www.scopus.com/inward/citedby.url?scp=85108300393&partnerID=8YFLogxK
U2 - 10.1016/j.bbrc.2021.06.034
DO - 10.1016/j.bbrc.2021.06.034
M3 - Article
C2 - 34166916
AN - SCOPUS:85108300393
SN - 0006-291X
VL - 567
SP - 183
EP - 189
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
ER -
