Multiple amino acids in the capsid structure of canine parvovirus coordinately determine the canine host range and specific antigenic and hemagglutination properties

Shwu Fen Chang, Jean Yves Sgro, Colin R. Parrish

Research output: Contribution to journalArticle

151 Citations (Scopus)

Abstract

Canine parvovirus (CPV) and feline panleukopenia virus (FPV) are over 98% similar in DNA sequence but have specific host range, antigenic, and hemagglutination (HA) properties which were located within the capsid protein gene. In vitro mutagenesis and recombination were used to prepare 16 different recombinant genomic clones, and viruses derived from those clones were analyzed for their in vitro host range, antigenic, and HA properties. The region of CPV from 59 to 91 map units determined the ability to replicate in canine cells. A complex series of interactions was observed among the individual sequence differences between 59 and 73 map units. The canine host range required that VP2 amino acids (aa) 93 and 323 both be the CPV sequence, and those two CPV sequences introduced alone into FPV greatly increased viral replication in canine cells. Changing any one of aa 93, 103, or 323 of CPV to the FPV sequence either greatly decreased replication in canine cells or resulted in an inviable plasmid. The Asn-Lys difference of aa 93 alone was responsible for the CPV-specific epitope recognized by monoclonal antibodies. An FPV-specific epitope was affected by aa 323. Amino acids 323 and 375 together determined the pH dependence of HA. Amino acids involved in the various specific properties were all around the threefold spikes of the viral particle.

Original languageEnglish
Pages (from-to)6858-6867
Number of pages10
JournalJournal of Virology
Volume66
Issue number12
Publication statusPublished - Dec 1992
Externally publishedYes

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Canine Parvovirus
Carnivore protoparvovirus 1
capsid
Capsid
Host Specificity
Feline Panleukopenia Virus
Hemagglutination
hemagglutination
host range
Canidae
Amino Acids
amino acids
dogs
Epitopes
Clone Cells
Aptitude
epitopes
Capsid Proteins
Individuality
Mutagenesis

ASJC Scopus subject areas

  • Immunology

Cite this

Multiple amino acids in the capsid structure of canine parvovirus coordinately determine the canine host range and specific antigenic and hemagglutination properties. / Chang, Shwu Fen; Sgro, Jean Yves; Parrish, Colin R.

In: Journal of Virology, Vol. 66, No. 12, 12.1992, p. 6858-6867.

Research output: Contribution to journalArticle

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