Mucoadhesive liposomes for intranasal immunization with an avian influenza virus vaccine in chickens

Chwei Jang Chiou, Li Ping Tseng, Ming Chung Deng, Pei Rong Jiang, Shang Li Tasi, Tze Wen Chung, Yi You Huang, Der Zen Liu

Research output: Contribution to journalArticlepeer-review

48 Citations (Scopus)


The aim of this study was to characterize a nasally delivered bioadhesive liposome using an inactivated H5N3 virus as a model antigen. Bioadhesive liposomes were developed using tremella (T) or xanthan gum (XG) as the bioadhesive polysaccharide. Using chickens as the target animal, we evaluated whether delivery of a bioadhesive liposomal influenza vaccine via a mucosal site of infection could improve vaccine effectiveness. 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS) cytotoxicity assays demonstrated that T, XG and liposomes were non toxic to chicken spleen macrophages. Enzyme-linked immunosorbent assay (ELISA) was used to determine the adjuvant effect of the bioadhesive liposomal-vaccines. Chickens immunized with a low dose (200 μL) of bioadhesive liposomal influenza vaccine had significantly higher mucosal and serum antibody levels (P <0.05). In addition, liposomes mixed with a low-viscosity bioadhesive gel used for nasal delivery resulted in superior antibody responses compared with liposomes mixed with a high-viscosity gel (P <0.05). This suggest that a low-viscosity gel mixed with liposomes is more suitable for nasal delivery, and that chickens elicit higher mucosal secretory immunoglobulin A (s-IgA) and serum IgG after two vaccinations.

Original languageEnglish
Pages (from-to)5862-5868
Number of pages7
Issue number29
Publication statusPublished - Oct 2009


  • Adjuvant
  • Intranasal
  • Liposome
  • Mucoadhesive
  • Newcastle disease virus
  • s-IgA

ASJC Scopus subject areas

  • Biomaterials
  • Bioengineering
  • Ceramics and Composites
  • Mechanics of Materials
  • Biophysics


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