MRNA decay factor AUF1 binds the internal ribosomal entry site of enterovirus 71 and inhibits virus replication

Jing Yi Lin, Mei Ling Li, Gary Brewer

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

AU-rich element binding factor 1 (AUF1) has a role in the replication cycles of different viruses. Here we demonstrate that AUF1 binds the internal ribosome entry site (IRES) of enterovirus 71 (EV71) and negatively regulates IRES-dependent translation. During EV71 infection, AUF1 accumulates in the cytoplasm where viral replication occurs, whereas AUF1 localizes predominantly in the nucleus in mock-infected cells. AUF1 knockdown in infected cells increases IRES activity and synthesis of viral proteins. Taken together, the results suggest that AUF1 interacts with the EV71 IRES to negatively regulate viral translation and replication.

Original languageEnglish
Article numbere103827
JournalPLoS One
Volume9
Issue number7
DOIs
Publication statusPublished - Jul 31 2014

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AU Rich Elements
Enterovirus
Viral Proteins
Virus Replication
virus replication
ribosomes
Viruses
deterioration
Enterovirus Infections
viral proteins
Cytoplasm
cytoplasm
cells
viruses
synthesis
Internal Ribosome Entry Sites
infection

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

MRNA decay factor AUF1 binds the internal ribosomal entry site of enterovirus 71 and inhibits virus replication. / Lin, Jing Yi; Li, Mei Ling; Brewer, Gary.

In: PLoS One, Vol. 9, No. 7, e103827, 31.07.2014.

Research output: Contribution to journalArticle

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