MRI tracking of polyethylene glycol-coated superparamagnetic iron oxide-labelled placenta-derived mesenchymal stem cells toward glioblastoma stem-like cells in a mouse model

Fei Ting Hsu, Zung Hang Wei, Yogi Chang Yo Hsuan, Willie Lin, Yu Chin Su, Chia Hui Liao, Chia Ling Hsieh

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Mesenchymal stem cells (MSCs) that display homing and infiltration properties towards tumor cells are a promising cellular targeting vector for brain tumor therapy but are limited to local-regional delivery in current preclinical models. Here, we investigated whether placenta-derived MSCs (P-MSCs) are a superior cellular vector for systemic targeting of glioblastoma stem-like cells (GSCs), with an imaging modality to real-time monitor the trafficking P-MSCs to glioblastoma sites. Results demonstrated that P-MSCs had greater migratory activity towards GSCs and across blood–brain barrier compared with bone marrow-derived MSCs, and this activity was enhanced by hypoxia precondition. Chemokine ligand 5 was identified as a chemoattractant responsible for the glioblastoma tropism of P-MSCs. Polyethylene glycol-coated superparamagnetic iron oxide (PEG–SPIO) was synthesized for cellular labelling and imaging P-MSCs, displaying high cellular uptake and no cytotoxic effect on P-MSCs cell proliferation or stemness property. The homing effects of intravenously administered PEG–SPIO-labelled P-MSCs towards intracerebral GSCs were able to be detected in mice models through T2-weighted magnetic resonance imaging (MRI). This study suggests the possibility of innovative systemic P-MSC-based cell therapy for aggressive GSCs, developing a state-of-the-art theranostic technique for real-time tracking of therapeutic P-MSCs tumor infiltration through cellular MRI.

Original languageEnglish
JournalArtificial Cells, Nanomedicine and Biotechnology
DOIs
Publication statusAccepted/In press - Jan 1 2018

Fingerprint

Magnetic resonance
Glioblastoma
Stem cells
Mesenchymal Stromal Cells
Iron oxides
Placenta
Polyethylene glycols
Stem Cells
Magnetic Resonance Imaging
Imaging techniques
Tumors
Infiltration
Cell proliferation
Labeling
Brain
Bone
Display devices
Ligands
Cells
ferric oxide

Keywords

  • cellular MRI
  • Glioblastoma stem-like cells
  • mesenchymal stem cell tumor-homing
  • placenta-derived mesenchymal stem cells
  • polyethylene glycol–coated superparamagnetic iron oxide

ASJC Scopus subject areas

  • Biotechnology
  • Medicine (miscellaneous)
  • Biomedical Engineering
  • Pharmaceutical Science

Cite this

MRI tracking of polyethylene glycol-coated superparamagnetic iron oxide-labelled placenta-derived mesenchymal stem cells toward glioblastoma stem-like cells in a mouse model. / Hsu, Fei Ting; Wei, Zung Hang; Hsuan, Yogi Chang Yo; Lin, Willie; Su, Yu Chin; Liao, Chia Hui; Hsieh, Chia Ling.

In: Artificial Cells, Nanomedicine and Biotechnology, 01.01.2018.

Research output: Contribution to journalArticle

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