Mouse embryo toxicity of IL-6 in peritoneal fluids from women with or without endometriosis

M. Y. Wu, S. U. Chen, K. H. Chao, C. D. Chen, Y. S. Yang, H. N. Ho

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Abstract

Background. To determine whether there is a factor (or factors) in the peritoneal fluid of endometriosis patients that impairs embryo growth and embryo implantation. Methods. Growth and development of two-cell mouse embryos which were cultured in media with peritoneal fluid from women with or without endometriosis and interleukin-1-beta (IL-1β), interleukin-6 (IL-6), and tumor necrosis factor-alpha (TNF-α) levels in conditioned media were measured. Results. The blastocyst rate in the non-endometriosis group was 46.4±31.1%, and that of the endometriosis group was 54.6±28.7%. Logistic regression analysis using the criteria of blastocyst development in 454 embryos, showed that the peritoneal fluid from endometriosis could promote (p=0.015) but IL-6 could arrest embryo growth to blastocyst (p=0.025). IL-1β and TNF-α levels had no significant effect on blastocyst formation. Conclusion. Peritoneal fluid from women with endometriosis was not toxic to mouse embryo development. However, IL-6 in the peritoneal fluid deteriorated the growth and development of mouse embryos.

Original languageEnglish
Pages (from-to)7-11
Number of pages5
JournalActa Obstetricia et Gynecologica Scandinavica
Volume80
Issue number1
DOIs
Publication statusPublished - Jan 30 2001
Externally publishedYes

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Keywords

  • Endometriosis
  • IL-1β
  • IL-6
  • Mouse embryos
  • Peritoneal fluid

ASJC Scopus subject areas

  • Obstetrics and Gynaecology

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