Motility and protein phosphorylation in healthy and asthenozoospermic sperm

Chying Chyuan Chan, Hao Ai Shui, Ching Hsiang Wu, Chih Yuan Wang, Guang Huan Sun, Han Min Chen, Gwo Jang Wu

Research output: Contribution to journalArticle

49 Citations (Scopus)

Abstract

The majority of male infertility results from poor sperm motility. A direct link between altered protein phosphorylation and aberrant sperm motility has not been established. To address this issue, sperm samples obtained from 20 donors with healthy sperm and 20 donors with aberrantly motile sperm were subjected to computer assisted semen analysis (CASA), proteomic analysis, Western blot, and immunofluorescent staining. Proteomic analysis identified 12 protein spots as having differential phosphorylation, including gamma-tubulin complex associated protein 2 (GCP2). Western blot and immunofluorescence demonstrated differential expression of gamma-tubulin between healthy and aberrantly motile sperm. In conclusion, hypophosphorylated proteins and reduced expression of gamma-tubulin may be associated with low motility sperm.

Original languageEnglish
Pages (from-to)5382-5386
Number of pages5
JournalJournal of Proteome Research
Volume8
Issue number11
DOIs
Publication statusPublished - Nov 6 2009
Externally publishedYes

Fingerprint

Molecular Motor Proteins
Phosphorylation
Spermatozoa
Sperm Motility
Tubulin
Proteomics
Proteins
Western Blotting
Semen Analysis
Male Infertility
Fluorescent Antibody Technique
Staining and Labeling

Keywords

  • Gamma-tubulin
  • Protein phosphorylation
  • Sperm motility
  • Spermatozoa infertility

ASJC Scopus subject areas

  • Biochemistry
  • Chemistry(all)

Cite this

Chan, C. C., Shui, H. A., Wu, C. H., Wang, C. Y., Sun, G. H., Chen, H. M., & Wu, G. J. (2009). Motility and protein phosphorylation in healthy and asthenozoospermic sperm. Journal of Proteome Research, 8(11), 5382-5386. https://doi.org/10.1021/pr9003932

Motility and protein phosphorylation in healthy and asthenozoospermic sperm. / Chan, Chying Chyuan; Shui, Hao Ai; Wu, Ching Hsiang; Wang, Chih Yuan; Sun, Guang Huan; Chen, Han Min; Wu, Gwo Jang.

In: Journal of Proteome Research, Vol. 8, No. 11, 06.11.2009, p. 5382-5386.

Research output: Contribution to journalArticle

Chan, CC, Shui, HA, Wu, CH, Wang, CY, Sun, GH, Chen, HM & Wu, GJ 2009, 'Motility and protein phosphorylation in healthy and asthenozoospermic sperm', Journal of Proteome Research, vol. 8, no. 11, pp. 5382-5386. https://doi.org/10.1021/pr9003932
Chan, Chying Chyuan ; Shui, Hao Ai ; Wu, Ching Hsiang ; Wang, Chih Yuan ; Sun, Guang Huan ; Chen, Han Min ; Wu, Gwo Jang. / Motility and protein phosphorylation in healthy and asthenozoospermic sperm. In: Journal of Proteome Research. 2009 ; Vol. 8, No. 11. pp. 5382-5386.
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