Morphometric study of glycine-immunoreactive neurons and terminals in the rat cuneate nucleus

J. H. Lue, W. F. Shieh, S. H. Chen, J. Y. Shieh, C. Y. Wen

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

The distribution of glycine-immunoreactive (glycine-IR) neurons and their associated axon terminals in the rat cuneate nucleus was studied using antiglycine postembedding immunoperoxidase labelling and immunogold staining, respectively. The immunoperoxidase-labelled glycine-IR neurons were widely distributed in the entire rostrocaudal extent of the nucleus. They made up 30.8% (9671/31368) of the neurons surveyed. Quantitative evaluation showed that the percentage of glycine-IR neurons in the caudal level was significantly higher than that in the middle and rostral levels. The glycine-IR neurons were small cells (mean area = 198 ± 1.9 μm 2, n = 2862) with ovoid or spindle-shaped somata. Statistical analysis showed that the size of the glycine-IR neurons in the rostral level was significantly smaller than that in the middle and caudal levels. Immunogold labelled glycine-IR terminals which contained predominantly pleomorphic synaptic vesicles were mostly small (mean area = 1.24 ± 0.03 μm 2, n = 286) and they constituted 24.7% (286/1158) of the total terminals surveyed. They formed axodendritic, axosomatic and axoaxonic synapses with unlabelled elements. It is suggested from this study that glycine is one of the major neurotransmitters involved in the depression of synaptic transmission in the cuneate nucleus.

Original languageEnglish
Pages (from-to)375-385
Number of pages11
JournalJournal of Anatomy
Volume191
Issue number3
DOIs
Publication statusPublished - Oct 1997
Externally publishedYes

Fingerprint

vesicle
glycine (amino acid)
Glycine
statistical analysis
neurons
Neurons
rats
synaptic transmission
Synaptic Vesicles
Presynaptic Terminals
Carisoprodol
synapse
neurotransmitters
evaluation
distribution
labelling
axons
Synaptic Transmission
Synapses
Neurotransmitter Agents

Keywords

  • Brainstem
  • Somatosensory system

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Anatomy

Cite this

Morphometric study of glycine-immunoreactive neurons and terminals in the rat cuneate nucleus. / Lue, J. H.; Shieh, W. F.; Chen, S. H.; Shieh, J. Y.; Wen, C. Y.

In: Journal of Anatomy, Vol. 191, No. 3, 10.1997, p. 375-385.

Research output: Contribution to journalArticle

Lue, J. H. ; Shieh, W. F. ; Chen, S. H. ; Shieh, J. Y. ; Wen, C. Y. / Morphometric study of glycine-immunoreactive neurons and terminals in the rat cuneate nucleus. In: Journal of Anatomy. 1997 ; Vol. 191, No. 3. pp. 375-385.
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