Monocyte chemotactic protein-1 in the migration of differentiated leukaemic cells toward alveolar epithelial cells

W. H. Tsai, C. H. Shih, C. C. Lin, C. K. Ho, F. C. Hsu, H. C. Hsu

Research output: Contribution to journalArticle

17 Citations (Scopus)


All-trans retinoic acid (ATRA) can induce acute respiratory distress syndrome in patients with acute promyelocytic leukaemia (APL). The current study investigated the role of monocyte chemotactic protein (MCP)-1 in the chemotactic transmigration of ATRA-treated NB4 (ATRA-NB4) APL cells toward A549 alveolar epithelial cells. NB4 and A549 cells were separately cultured with ATRA and/or dexamethasone (DEX). ATRA-NB4 cells were then placed in an upper insert and co-incubated with A549 cells or their conditioned medium (CM) located in a lower plate to test their transmigration activity. ATRA stimulated NB4 cells to transmigrate toward the A549 cells. The secretion of MCP-1 was enhanced by ATRA treatment in both A549 and NB4 cells. The binding assay demonstrated that ATRA-NB4 cells bound MCP-1. Pre-treatment of both CM-A549 cells with antibodies against MCP-1 and of ATRA-NB4 cells with antibodies against MCP-1 receptors reduced ATRA-NB4 cell transmigration. DEX did not suppress MCP-1 secretion and transmigration in ATRA-NB4 cells, although when applied to A549 cells, MCP-1 secretion was suppressed and ATRA-NB4 cell transmigration was attenuated. Monocyte chemotactic protein-1 secreted from alveolar epithelial cells plays an important role in the cell-cell interaction involved in the chemotactic transmigration of all-trans retinoic acidtreated acute promyelocytic leukaemia cells toward alveolar epithelial cells. Copyright

Original languageEnglish
Pages (from-to)957-962
Number of pages6
JournalEuropean Respiratory Journal
Issue number5
Publication statusPublished - May 2008



  • Acute respiratory distress syndrome
  • All-trans retinoic acid
  • Chemokine
  • Leukocyte infiltration
  • Monocyte chemotactic protein-1
  • Retinoid acid syndrome

ASJC Scopus subject areas

  • Pulmonary and Respiratory Medicine
  • Medicine(all)

Cite this