Monoclonal antibody-functionalized mesoporous silica nanoparticles (MSN) for selective targeting breast cancer cells

Chih Pin Tsai, Chao Yu Chen, Yann Hung, Fu Hsiung Chang, Chung Yuan Mou

Research output: Contribution to journalArticlepeer-review

222 Citations (Scopus)

Abstract

In this work, we conjugate anti-HER2/neu mAb (monoclonal antibody) to green fluorescent dye loaded mesoporous silica nanoparticles (Her-Dye@MSN) through a polyethylene glycol (MW = 5000) spacer. We examine their targeting properties toward HER2/neu over-expressing breast cancer cells and their internalization into the cells. Her-Dye@MSN nanoparticles exhibit a high targeting efficiency and the specific targeting capability is strongly affected by the mAb density on the Her-Dye@MSN. The time-course of competitive experiments with free antibody indicates that Her-Dye@MSN serves as a multivalent ligand. Moreover, Her-Dye@MSNs are internalized into cells through a receptor-mediated endocytosis and may escape from endosome to cytosol. The results reported here further support the potential of MSNs as a multifunctional smart nanocarriers for cell imaging and drug delivery.

Original languageEnglish
Pages (from-to)5737-5743
Number of pages7
JournalJournal of Materials Chemistry
Volume19
Issue number32
DOIs
Publication statusPublished - 2009
Externally publishedYes

ASJC Scopus subject areas

  • Chemistry(all)
  • Materials Chemistry

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