Monoamine oxidase B and free radical scavenging activities of natural flavonoids in Melastoma candidum D. Don

M. H. Lee, R. D. Lin, L. Y. Shen, Ling-Ling Yang, K. Y. Yen, W. C. Hou

Research output: Contribution to journalArticle

69 Citations (Scopus)

Abstract

Monoamine oxidase type B (MAO-B) activity and free radicals are elevated in certain neurological diseases. Four natural flavonoids, quercitrin, isoquercitrin, rutin, and quercetin, were isolated for the first time from the leaves of Melastoma candidum D. Don. They exhibited an inhibitory effect on MAO-B. These potent flavonoids were purified using bioassay-guided fractionation and were separated by Diaion, Sephadex LH-20, and MCI CHP20P columns. The IC50 values of the four potent flavonoids, quercitrin, isoquercitrin, rutin, and quercetin on monoamine oxidase were 19.06, 11.64, 3.89, and 10.89 μM and enzyme kinetics analysis revealed apparent inhibition constants (Kj) of 21.01, 2.72, 1.83, and 7.95 μM, respectively, on the substrate, benzylamine. The four potent compounds also exhibited hydroxyl radical scavenging activity as determined using a spin trapping electron spin resonance method. This suggests that the four flavonoids from M. candidum possess both MAO-B inhibitory and free radical scavenging activities. These important properties may be used for preventing some neurodegenerative diseases in the future.

Original languageEnglish
Pages (from-to)5551-5555
Number of pages5
JournalJournal of Agricultural and Food Chemistry
Volume49
Issue number11
DOIs
Publication statusPublished - 2001

Fingerprint

Melastoma candidum
amine oxidase (flavin-containing)
Scavenging
Monoamine Oxidase
Flavonoids
Free Radicals
flavonoids
isoquercitrin
quercitrin
Rutin
Quercetin
rutin
quercetin
Neurodegenerative diseases
Spin Trapping
Enzyme inhibition
Enzyme kinetics
enzyme kinetics
Bioassay
electron paramagnetic resonance spectroscopy

Keywords

  • Hydroxyl radical scavenging
  • Isoquercitrin
  • Melastoma candidum
  • Monoamine oxidase B
  • Quercetin
  • Quercitrin
  • Rutin

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Food Science
  • Chemistry (miscellaneous)

Cite this

Monoamine oxidase B and free radical scavenging activities of natural flavonoids in Melastoma candidum D. Don. / Lee, M. H.; Lin, R. D.; Shen, L. Y.; Yang, Ling-Ling; Yen, K. Y.; Hou, W. C.

In: Journal of Agricultural and Food Chemistry, Vol. 49, No. 11, 2001, p. 5551-5555.

Research output: Contribution to journalArticle

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